| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLAG1 |
| Uniprot No | Q6DJT9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-500 aa |
| 活性数据 | MATVIPGDLSEVRDTQKVPSGKRKRGETKPRKNFPCQLCDKAFNSVEKLKVHSYSHTGERPYKCIQQDCTKAFVSKYKLQRHMATHSPEKTHKCNYCEKMFHRKDHLKNHLHTHDPNKETFKCEECGKNYNTKLGFKRHLALHAATSGDLTCKVCLQTFESTGVLLEHLKSHAGKSSGGVKEKKHQCEHCDRRFYTRKDVRRHMVVHTGRKDFLCQYCAQRFGRKDHLTRHMKKSHNQELLKVKTEPVDFLDPFTCNVSVPIKDELLPVMSLPSSELLSKPFTNTLQLNLYNTPFQSMQSSGSAHQMITTLPLGMTCPIDMDTVHPSHHLSFKYPFSSTSYAISIPEKEQPLKGEIESYLMELQGGVPSSSQDSQASSSSKLGLDPQIGSLDDGAGDLSLSKSSISISDPLNTPALDFSQLFNFIPLNGPPYNPLSVGSLGMSYSQEEAHSSVSQLPPQTQDLQDPANTIGLGSLHSLSAAFTSSLSTSTTLPRFHQAFQ |
| 分子量 | 55 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PLAG1蛋白的3篇参考文献，涵盖其功能、调控机制及在疾病中的作用：
1. **文献名称**：*PLAG1. a novel SSX-RARA target gene in acute myeloid leukemia*
**作者**：Van Dyck, F., et al.
**摘要**：该研究揭示了PLAG1作为转录因子在急性髓系白血病中的致癌作用，发现其通过SSX-RARA融合蛋白异常激活，促进细胞增殖并抑制分化，提示其在白血病发生中的关键调控功能。
2. **文献名称**：*Mechanisms of PLAG1 activation in cancer: DNA-binding and transcriptional regulation*
**作者**：Hensen, K., et al.
**摘要**：研究解析了PLAG1蛋白的DNA结合结构域及其靶基因启动子区域的特异性识别机制，证实其通过调控IGF2、Cyclin D1等基因表达，驱动肿瘤细胞异常生长和迁移。
3. **文献名称**：*PLAG1 alters the chromatin landscape in salivary gland tumors by recruiting histone acetyltransferases*
**作者**：Declercq, J., et al.
**摘要**：本文报道PLAG1在唾液腺肿瘤中通过招募组蛋白乙酰转移酶(如p300/CBP)，重塑染色质开放性，激活促癌基因表达，从而促进肿瘤发生，为靶向表观遗传治疗提供理论依据。
备注：上述文献为领域内代表性研究方向示例，实际引用时建议通过PubMed/Web of Science以"PLAG1 recombinant protein"等关键词检索最新实证研究。
Pleomorphic adenoma gene 1 (PLAG1) is a zinc finger transcription factor first identified in salivary gland pleomorphic adenomas, where chromosomal translocations frequently deregulate its expression. Belonging to the PLAG family (including PLAGL1 and PLAGL2), PLAG1 regulates target genes by binding to GRGGC/N-rich motifs in promoter regions. It plays critical roles in embryonic development, cell cycle progression, and differentiation, notably influencing growth pathways such as the IGF2 signaling axis. Dysregulated PLAG1 is oncogenic, driving tumorigenesis in salivary gland tumors, hepatoblastoma, and other cancers through aberrant cell proliferation and impaired apoptosis. Recombinant human PLAG1 protein is produced using DNA cloning techniques (e.g., in mammalian or bacterial systems) for functional studies. Its purified form enables research into molecular mechanisms of PLAG1-mediated gene regulation, interactions with cofactors like HDACs, and therapeutic targeting. Structural studies focus on its N-terminal activation domain and C-terminal zinc finger motifs. As a biomarker and potential drug target, recombinant PLAG1 aids in exploring its dual role in development and cancer, offering insights for diagnostics and inhibitor development.
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