| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLB1 |
| Uniprot No | Q6P1J6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423 aa |
| 活性数据 | MPITCPTQNEPFLRTPRNSNYTYPIKPAIENWGSDFLCTEWKASNSVPTSVHQLRPADIKVVAALGDSLTTAVGARPNNSSDLPTSWRGLSWSIGGDGNLETHTTLPNILKKFNPYLLGFSTSTWEGTAGLNVAAEGARARDMPAQAWDLLERMKNSPDINLEKDWKLVTLFIGVNDLCHYCENPEAHLATEYVQHIQQALDILSEELPRAFVNVVEVMELASLYQGQGGKCAMLAAQNNCTCLRHSQSSLEKQELKKVNWNLQHGISSFSYWHQYTQREDFAVVVQPFFQNTLTPLNERGDTDLTFFSEDCFHFSDRGHAEMAIALWNNMLEPVGRKTTSNNFTHSRAKLKCPSPESPYLYTLRNSRLLPDQAEEAPEVLYWAVPVAAGVGLVVGIIGTVVWRCRRGGRREDPPMSLRTVAL |
| 分子量 | 73.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PLB1(Phospholipase B1)蛋白的3-4条参考文献示例及其摘要概括：
1. **文献名称**：*Recombinant human PLB1 expression and its role in inflammatory response*
**作者**：Zhang Y., et al.
**摘要**：研究通过哺乳动物细胞系统成功表达了重组人PLB1蛋白，并证明其在脂质介质(如花生四烯酸)生成中起关键作用，进一步揭示了PLB1通过调节磷脂代谢加剧炎症反应的分子机制。
2. **文献名称**：*Structural and functional characterization of PLB1 in lung epithelial cells*
**作者**：Hsu L.C., et al.
**摘要**：解析了重组PLB1蛋白的晶体结构，表明其具有双重酶活性(磷脂酶A2和溶血磷脂酶)，并利用基因敲除模型发现PLB1缺失会抑制脂质过氧化产物积累，改善哮喘模型中的气道高反应性。
3. **文献名称**：*PLB1-mediated lipid remodeling promotes cancer cell invasion*
**作者**：Chen X., et al.
**摘要**：通过体外重组蛋白实验和临床样本分析，发现PLB1过表达通过水解细胞膜磷脂生成溶血磷脂酸(LPA)，激活下游信号通路，显著增强黑色素瘤细胞的迁移和侵袭能力。
4. **文献名称**：*Recombinant PLB1 as a biomarker for fungal infections*
**作者**：Garcia-Rodas R., et al.
**摘要**：研究利用重组人PLB1蛋白开发新型ELISA检测方法，验证其在隐球菌感染患者血清中的特异性抗体水平升高，提示其作为侵袭性真菌病诊断标志物的潜力。
注：以上内容为示例性整理，具体文献请通过PubMed或Web of Science等数据库以“recombinant human PLB1 protein”等关键词检索确认。
Recombinant human phospholipase B1 (PLB1) is a genetically engineered protein derived from the human *PLB1* gene, which encodes a multifunctional enzyme with both phospholipase A2 and lysophospholipase activities. Primarily expressed in tissues like the lungs, intestines, and immune cells, PLB1 plays a critical role in lipid metabolism by hydrolyzing phospholipids into bioactive mediators, influencing membrane remodeling, cell signaling, and inflammatory responses. Its structure includes a conserved catalytic domain and regulatory regions that mediate interactions with cellular membranes or cofactors. Dysregulation of PLB1 has been linked to pathologies such as acute respiratory distress syndrome (ARDS), bacterial infections, and cancer metastasis, highlighting its dual role in maintaining membrane homeostasis and contributing to disease progression.
Recombinant PLB1 is produced via bacterial, yeast, or mammalian expression systems, enabling studies on its enzymatic mechanisms, substrate specificity, and therapeutic potential. It serves as a valuable tool for investigating lipid-driven processes, drug discovery (e.g., anti-inflammatory or antimicrobial agents), and understanding host-pathogen interactions, as some pathogens exploit host phospholipases for invasion. Despite its biological significance, PLB1's regulation and context-dependent functions remain under exploration, with ongoing research aiming to clarify its tissue-specific roles and therapeutic targeting strategies.
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