| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLA2G6 |
| Uniprot No | O60733 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-752 aa |
| 活性数据 | MQFFGRLVNTFSGVTNLFSNPFRVKEVAVADYTSSDRVREEGQLILFQNTPNRTWDCVLVNPRNSQSGFRLFQLELEADALVNFHQYSSQLLPFYESSPQVLHTEVLQHLTDLIRNHPSWSVAHLAVELGIRECFHHSRIISCANCAENEEGCTPLHLACRKGDGEILVELVQYCHTQMDVTDYKGETVFHYAVQGDNSQVLQLLGRNAVAGLNQVNNQGLTPLHLACQLGKQEMVRVLLLCNARCNIMGPNGYPIHSAMKFSQKGCAEMIISMDSSQIHSKDPRYGASPLHWAKNAEMARMLLKRGCNVNSTSSAGNTALHVAVMRNRFDCAIVLLTHGANADARGEHGNTPLHLAMSKDNVEMIKALIVFGAEVDTPNDFGETPTFLASKIGRQLQDLMHISRARKPAFILGSMRDEKRTHDHLLCLDGGGVKGLIIIQLLIAIEKASGVATKDLFDWVAGTSTGGILALAILHSKSMAYMRGMYFRMKDEVFRGSRPYESGPLEEFLKREFGEHTKMTDVRKPKVMLTGTLSDRQPAELHLFRNYDAPETVREPRFNQNVNLRPPAQPSDQLVWRAARSSGAAPTYFRPNGRFLDGGLLANNPTLDAMTEIHEYNQDLIRKGQANKVKKLSIVVSLGTGRSPQVPVTCVDVFRPSNPWELAKTVFGAKELGKMVVDCCTDPDGRAVDRARAWCEMVGIQYFRLNPQLGTDIMLDEVSDTVLVNALWETEVYIYEHREEFQKLIQLLLSP |
| 分子量 | 110.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人PLA2G6蛋白相关的研究文献概览：
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1. **Structural Insights into Human PLA2G6 Function and Pathogenic Mutations**
*作者：Engel, L. A., et al.*
摘要：解析了重组人PLA2G6的晶体结构，发现其钙依赖性磷脂酶活性区域的构象变化与神经退行性疾病(如婴儿神经轴突营养不良)相关突变直接干扰脂质代谢功能。
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2. **Enzymatic Characterization of Recombinant PLA2G6 in Lipid Peroxidation Pathways**
*作者：Mori, A., & Shimizu, T.*
摘要：通过体外重组表达PLA2G6蛋白，证实其在细胞膜磷脂水解和铁死亡调控中的作用，并揭示酶活性丧失导致线粒体膜稳定性下降的分子机制。
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3. **PLA2G6 Mutations Disrupt Autophagy-Lysosome Pathways in Parkinsonian Models**
*作者：Zhao, Y., et al.*
摘要：利用重组突变型PLA2G6蛋白构建帕金森病模型，发现PLA2G6功能缺陷引发α-突触核蛋白异常聚集，并通过自噬-溶酶体通路障碍加剧神经元退行性病变。
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4. **PLA2G6-associated Neurodegeneration: Functional Rescue via Recombinant Protein Therapy**
*作者：Kong, S. M., et al.*
摘要：在小鼠模型中验证重组人PLA2G6蛋白递送可部分恢复神经元脂质稳态，为PLA2G6相关疾病的基因治疗提供实验依据。
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注：以上文献信息为示例性质，实际研究请通过PubMed或专业数据库检索。
Phospholipase A2 Group VI (PLA2G6), also known as calcium-independent phospholipase A2-beta (iPLA2-β), is a member of the phospholipase A2 superfamily. This 85-88 kDa enzyme is encoded by the PLA2G6 gene in humans and functions as a calcium-dependent cytosolic phospholipase. It plays a critical role in phospholipid metabolism by catalyzing the hydrolysis of membrane phospholipids into free fatty acids and lysophospholipids, influencing lipid mediator production, membrane homeostasis, and inflammatory responses.
PLA2G6 contains a catalytic domain and multiple functional motifs, including ankyrin repeats and a C2-like domain, which regulate its membrane association and enzymatic activity. It is widely expressed in tissues, particularly in the brain, where it maintains neuronal membrane integrity and participates in signal transduction.
Mutations in PLA2G6 are linked to neurodegenerative disorders, including infantile neuroaxonal dystrophy (INAD) and Parkinson’s disease, attributed to defective lipid remodeling, oxidative stress, and mitochondrial dysfunction. Recombinant human PLA2G6 protein is produced in heterologous systems (e.g., CHO or insect cells) for functional studies, enabling exploration of its enzymatic mechanisms, pathological roles, and interactions with cellular pathways. Researchers utilize it to model diseases, screen therapeutic compounds targeting PLA2G6-related pathways, and develop gene therapies aimed at restoring its activity in mutation-associated conditions. Its recombinant form retains calcium-dependent hydrolytic activity, making it a vital tool for studying neuroinflammatory and degenerative processes.
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