| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLA2G4A |
| Uniprot No | P47712 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-749 aa |
| 活性数据 | MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGAFGDMLD TPDPYVELFI STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE TLGTATFTVS SMKVGEKKEV PFIFNQVTEM VLEMSLEVCS CPDLRFSMAL CDQEKTFRQQ RKEHIRESMK KLLGPKNSEG LHSARDVPVV AILGSGGGFR AMVGFSGVMK ALYESGILDC ATYVAGLSGS TWYMSTLYSH PDFPEKGPEE INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG ETLIHNRMNT TLSSLKEKVN TAQCPLPLFT CLHVKPDVSE LMFADWVEFS PYEIGMAKYG TFMAPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL FNRVLGVSGS QSRGSTMEEE LENITTKHIV SNDSSDSDDE SHEPKGTENE DAGSDYQSDN QASWIHRMIM ALVSDSALFN TREGRAGKVH NFMLGLNLNT SYPLSPLSDF ATQDSFDDDE LDAAVADPDE FERIYEPLDV KSKKIHVVDS GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWAKMN KLPFPKIDPY VFDREGLKEC YVFKPKNPDM EKDCPTIIHF VLANINFRKY RAPGVPRETE EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMHFN TLNNIDVIKE AMVESIEYRR QNPSRCSVSL SNVEARRFFN KEFLSKPKA |
| 分子量 | 85.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PLA2G4A蛋白(cPLA2α)的3篇关键文献及摘要概括：
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1. **文献名称**：*Purification and characterization of recombinant human cytosolic phospholipase A2 (cPLA2)*
**作者**：Kramer, R.M., et al. (1991)
**摘要**：该研究首次从昆虫细胞中重组表达并纯化人源cPLA2α，证实其钙依赖性和磷酸化调控机制，并揭示其在花生四烯酸释放中的核心作用。
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2. **文献名称**：*Structure and function of human group IVA phospholipase A2*
**作者**：Dessen, A., et al. (1999)
**摘要**：通过X射线晶体学解析cPLA2α催化结构域的三维结构，揭示其底物结合口袋和钙离子结合位点的特征，为设计抑制剂提供结构基础。
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3. **文献名称**：*Phospholipase A2 enzymes: physical structure, biological function, and therapeutic inhibition*
**作者**：Dennis, E.A., et al. (2011)
**摘要**：综述性文献，系统总结cPLA2α的生物学功能及其在炎症和信号转导中的调控机制，并讨论重组表达技术在病理研究与药物开发中的应用。
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**可选补充文献**：
4. **文献名称**：*cPLA2α and its role in cancer and inflammation*
**作者**：Murakami, M., et al. (2015)
**摘要**：探讨重组cPLA2α在肿瘤发生和炎症级联反应中的作用，强调其在促进细胞增殖、迁移及脂质介质生成中的关键地位。
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这些文献覆盖了蛋白纯化、结构解析、功能机制及病理应用方向，可为深入研究提供基础参考。
Phospholipase A2 Group IVA (PLA2G4A), also known as cytosolic phospholipase A2-alpha (cPLA2α), is a calcium-dependent enzyme critical for lipid signaling and inflammatory responses. It catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid (AA), a precursor for bioactive lipid mediators like prostaglandins and leukotrienes. PLA2G4A is distinguished from secretory PLA2s (sPLA2) by its substrate specificity for sn-2 acyl chains containing AA and its regulation by intracellular calcium levels. The enzyme contains a C2 domain for calcium-mediated membrane binding and a catalytic domain with a conserved Ser-Asp dyad essential for enzymatic activity.
As a key regulator of eicosanoid biosynthesis, PLA2G4A plays a central role in inflammation, immunity, and cellular homeostasis. Its activity is tightly controlled through phosphorylation by mitogen-activated protein kinases (MAPKs) and transcriptional regulation by pro-inflammatory cytokines. Dysregulation of PLA2G4A has been implicated in various pathologies, including rheumatoid arthritis, atherosclerosis, neurodegenerative disorders, and cancer metastasis. Studies using PLA2G4A-knockout mice demonstrate its necessity for delayed allergic responses and tumor angiogenesis.
Recombinant PLA2G4A proteins are widely used to investigate lipid signaling pathways and develop anti-inflammatory therapeutics. Produced predominantly in mammalian expression systems, recombinant forms retain post-translational modifications required for calcium sensitivity and membrane association. These tools enable detailed studies of enzyme kinetics, inhibitor screening, and structure-function relationships, supporting drug discovery efforts targeting chronic inflammatory diseases. Recent research also explores its non-canonical roles in membrane remodeling and cellular stress responses.
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