| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKP3 |
| Uniprot No | Q9Y446 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-797 aa |
| 活性数据 | MQDGNFLLSALQPEAGVCSLALPSDLQLDRRGAEGPEAERLRAARVQEQVRARLLQLGQQPRHNGAAEPEPEAETARGTSRGQYHTLQAGFSSRSQGLSGDKTSGFRPIAKPAYSPASWSSRSAVDLSCSRRLSSAHNGGSAFGAAGYGGAQPTPPMPTRPVSFHERGGVGSRADYDTLSLRSLRLGPGGLDDRYSLVSEQLEPAATSTYRAFAYERQASSSSSRAGGLDWPEATEVSPSRTIRAPAVRTLQRFQSSHRSRGVGGAVPGAVLEPVARAPSVRSLSLSLADSGHLPDVHGFNSYGSHRTLQRLSSGFDDIDLPSAVKYLMASDPNLQVLGAAYIQHKCYSDAAAKKQARSLQAVPRLVKLFNHANQEVQRHATGAMRNLIYDNADNKLALVEENGIFELLRTLREQDDELRKNVTGILWNLSSSDHLKDRLARDTLEQLTDLVLSPLSGAGGPPLIQQNASEAEIFYNATGFLRNLSSASQATRQKMRECHGLVDALVTSINHALDAGKCEDKSVENAVCVLRNLSYRLYDEMPPSALQRLEGRGRRDLAGAPPGEVVGCFTPQSRRLRELPLAADALTFAEVSKDPKGLEWLWSPQIVGLYNRLLQRCELNRHTTEAAAGALQNITAGDRRWAGVLSRLALEQERILNPLLDRVRTADHHQLRSLTGLIRNLSRNARNKDEMSTKVVSHLIEKLPGSVGEKSPPAEVLANIIAVLNNLVGASPIAARDLLYFDGLRKLIFIKKKRDSPDSEKSSRAASSLLANLWQYNKLHRDFRAKGYRKEDFLGP |
| 分子量 | 113.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PKP3蛋白的3篇参考文献示例(文献为假设性示例，仅供参考)：
1. **文献名称**：*Recombinant human PKP3 expression and purification for structural analysis*
**作者**：Smith J, et al.
**摘要**：该研究报道了通过大肠杆菌表达系统高效表达重组人PKP3蛋白，并优化纯化方法获得高纯度蛋白，通过X射线晶体学解析其三维结构，为研究PKP3的分子功能提供基础。
2. **文献名称**：*PKP3 regulates cell adhesion via interaction with desmosomal components in keratinocytes*
**作者**：Li X, et al.
**摘要**：利用重组人PKP3蛋白进行体外结合实验，证实其与桥粒蛋白(如desmoplakin)的相互作用，阐明PKP3在上皮细胞间黏附及皮肤屏障功能中的关键作用。
3. **文献名称**：*Role of recombinant PKP3 in modulating Wnt/β-catenin signaling in cancer cells*
**作者**：Garcia R, et al.
**摘要**：研究发现重组PKP3蛋白通过调控Wnt信号通路影响肿瘤细胞迁移，并揭示其在癌症转移中的潜在分子机制，为靶向治疗提供新思路。
(注：以上文献为示例，实际研究需根据具体数据库检索。)
Plakophilin 3 (PKP3) is a member of the plakophilin family of armadillo (ARM)-repeat proteins, primarily known for their role in cell-cell adhesion and structural integrity. As a core component of desmosomal plaques, PKP3 interacts with desmosomal cadherins (e.g., desmocollin and desmoglein) and intermediate filament proteins, facilitating the assembly and stabilization of desmosomes—specialized junctions critical for epithelial and cardiac tissue integrity. PKP3 is widely expressed in stratified and simple epithelia, where it supports tissue resilience against mechanical stress and regulates signaling pathways involved in cell proliferation, differentiation, and apoptosis.
Recombinant human PKP3 protein is engineered using bacterial or mammalian expression systems to produce purified, biologically active forms of the protein for research. It typically retains functional domains, including nine ARM repeats that mediate protein-protein interactions, enabling studies on its binding partners and mechanistic roles. Research highlights PKP3's dual role in cancer: it may act as a tumor suppressor by maintaining epithelial homeostasis or as an oncogenic driver in certain contexts, promoting cell migration, invasion, and metastasis through pathways like Wnt/β-catenin and EGFR signaling. Dysregulation of PKP3 has been linked to carcinomas (e.g., breast, lung, skin) and skin disorders (e.g., ectodermal dysplasia).
Recombinant PKP3 is instrumental in deciphering desmosome dynamics, disease mechanisms, and therapeutic targeting. It also serves as an antigen for antibody development or a control in diagnostic assays. Its applications span molecular biology, cancer research, and regenerative medicine.
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