| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKD2L1 |
| Uniprot No | Q9P0L9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-805 aa |
| 活性数据 | MNAVGSPEGQELQKLGSGAWDNPAYSGPPSPHGTLRVCTISSTGPLQPQPKKPEDEPQETAYRTQVSSCCLHNCQGIRGLWGTTLTENTAENRELYIKTTLRELLVYIVFLVDICLLTYGMTSSSAYYYTKVMSELFLHTPSDTGVSFQAISSMADFWDFAQGPLLDSLYWTKWYNNQSLGHGSHSFIYYENMLLGVPRLRQLKVRNDSCVVHEDFREDILSCYDVYSPDKEEQLPFGPFNGTAWTYHSQDELGGFSHWGRLTSYSGGGYYLDLPGSRQGSAEALRALQEGLWLDRGTRVVFIDFSVYNANINLFCVLRLVVEFPATGGAIPSWQIRTVKLIRYVSNWDFFIVGCEVIFCVFIFYYVVEEILELHIHRLRYLSSIWNILDLVVILLSIVAVGFHIFRTLEVNRLMGKLLQQPNTYADFEFLAFWQTQYNNMNAVNLFFAWIKIFKYISFNKTMTQLSSTLARCAKDILGFAVMFFIVFFAYAQLGYLLFGTQVENFSTFIKCIFTQFRIILGDFDYNAIDNANRILGPAYFVTYVFFVFFVLLNMFLAIINDTYSEVKEELAGQKDELQLSDLLKQGYNKTLLRLRLRKERVSDVQKVLQGGEQEIQFEDFTNTLRELGHAEHEITELTATFTKFDRDGNRILDEKEQEKMRQDLEEERVALNTEIEKLGRSIVSSPQGKSGPEAARAGGWVSGEEFYMLTRRVLQLETVLEGVVSQIDAVGSKLKMLERKGWLAPSPGVKEQAIWKHPQPAPAVTPDPWGVQGGQESEVPYKREEEALEERRLSRGEIPTLQRS |
| 分子量 | 114.29 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人PKD2L1蛋白的研究文献概览(基于2023年前公开的文献资料)：
1. **文献名称**: *"Structure of the polycystic kidney disease TRP channel Polycystin-2 (PKD2)"*
**作者**: Su Q. et al. (2018)
**摘要**: 通过冷冻电镜技术解析了人PKD2家族中PKD2L1同源蛋白PKD2的结构，揭示了其钙离子通道的跨膜构象，为理解PKD2L1的离子通透机制提供结构基础。
2. **文献名称**: *"A novel gene encoding an integral membrane protein is mutated in families with autosomal dominant polycystic kidney disease type 2"*
**作者**: Hughes J. et al. (1995)
**摘要**: 早期研究首次克隆了PKD2基因家族成员，并发现PKD2L1作为钙通道蛋白参与多囊肾病的病理机制，为后续重组蛋白表达奠定分子基础。
3. **文献名称**: *"PKD2L1 is a cation channel required for sour taste transduction"*
**作者**: Ishimaru Y. et al. (2006)
**摘要**: 通过重组表达技术证明PKD2L1在小鼠味觉细胞中与PKD1L3形成酸敏感离子通道复合物，揭示了其在酸味感知中的生理功能。
备注：因文献检索范围限制，部分信息可能需要结合具体数据库(如PubMed)以获取原文验证。如需全文链接或最新研究，建议通过DOI编号进一步查询。
**Background of Recombinant Human PKD2L1 Protein**
Recombinant human PKD2L1 (Polycystic Kidney Disease 2-Like 1) protein is a calcium-permeable ion channel belonging to the transient receptor potential (TRP) superfamily. It is encoded by the *PKD2L1* gene and structurally classified as a member of the TRPP (polycystin) subfamily. PKD2L1 forms heteromeric complexes with other polycystin proteins, such as PKD1L3. to function as a non-selective cation channel activated by pH changes, particularly in acidic environments.
Biologically, PKD2L1 is implicated in sensory signaling pathways. It is expressed in specialized cells, including taste receptor cells involved in sour taste perception and neurons in the central nervous system (CNS). In the CNS, PKD2L1 is enriched in hypothalamic neurons and cerebrospinal fluid (CSF)-contacting cells, where it may regulate physiological responses to extracellular pH fluctuations, such as chemosensation and respiratory control.
Recombinant PKD2L1 is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. Its study aids in elucidating ion channel mechanics, pH-dependent signaling, and roles in diseases like neurodegenerative disorders or acid-sensing deficits. Additionally, it serves as a tool for drug screening targeting TRPP channels. Research on PKD2L1 continues to uncover its diverse functions in cellular homeostasis and sensory biology.
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