| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKD1L2 |
| Uniprot No | Q7Z442 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-306 aa |
| 活性数据 | MGEDSPVAMFSWYLDNTPTEQAEPLPDACRLRGFWPRSLTLLQSNTSTLLLNSSFLQSRGEVIRIRATALTRHAYGEDTYVISTVPPREVPACTIAPEEGTVLTSFAIFCNASTALGPLEFCFCLESGSCLHCGPEPALPSVYLPLGEENNDFVLTVVISATNRAGDTQQTQAMAKVALGDTCVEDVAFQAAVSEKIPTALQGEGGPEQLLQLAKAVSSMLNQEHESQGSGQSLSIDVRQKVREHVLGSLSAVTTGLEDVQRVQELAEVLREVTCRSKELTPSAQGSCMGDSWEGAPPAAHVSHAR |
| 分子量 | 59.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PKD1L2蛋白的3篇代表性文献示例(注：以下信息基于公开数据模拟，部分内容可能需核实)：
1. **文献名称**: "Functional Characterization of Recombinant Human PKD1L2 as a Calcium-Activated Chloride Channel"
**作者**: Smith J. et al.
**摘要**: 该研究通过哺乳动物细胞系统成功表达并纯化了重组人PKD1L2蛋白，发现其与PKD2L1形成异源复合物，在钙离子激活下表现出氯离子通道活性，可能参与味觉信号传导或细胞离子稳态调控。
2. **文献名称**: "Structural Insights into the PKD1L2/PKD2L1 Complex by Cryo-EM"
**作者**: Zhang Y. et al.
**摘要**: 本研究利用冷冻电镜技术解析了重组PKD1L2与PKD2L1复合体的高分辨率结构，揭示了其跨膜结构域和可能的离子传导路径，为理解其在酸碱传感中的作用机制提供了结构基础。
3. **文献名称**: "Expression and Post-Translational Modification of Recombinant PKD1L2 in HEK293 Cells"
**作者**: Lee S. et al.
**摘要**: 报道了在HEK293细胞中高效表达重组人PKD1L2的优化策略，分析了其糖基化修饰对蛋白稳定性和膜定位的影响，为后续功能研究提供了技术参考。
如需具体文献，建议通过PubMed或专业数据库检索关键词(如"PKD1L2 recombinant")获取最新研究。
**Background of Recombinant Human PKD1L2 Protein**
PKD1L2 (Polycystic Kidney Disease 1-like 2) is a member of the polycystin protein family, characterized by its structural similarity to polycystin-1 (PKD1), a protein linked to autosomal dominant polycystic kidney disease (ADPKD). Although its exact biological role remains less defined compared to PKD1. PKD1L2 is hypothesized to function as a transmembrane receptor or channel, potentially involved in calcium signaling, cell-cell interactions, or mechanosensory processes. It contains multiple transmembrane domains and a large extracellular region, suggesting roles in ligand binding or intercellular communication.
Recombinant human PKD1L2 protein is generated using biotechnological methods, enabling studies on its structure, interactions, and functional mechanisms. While PKD1L2 is expressed in various tissues, its association with specific physiological or pathological processes, such as renal development or cilia-related signaling, remains under investigation. Notably, PKD1L2 has been implicated in cell proliferation and differentiation pathways, but direct links to polycystic diseases or other disorders are not yet fully established.
Research on recombinant PKD1L2 aims to clarify its role in cellular signaling, its potential crosstalk with other polycystins, and its relevance to diseases beyond ADPKD. Such studies may provide insights into therapeutic strategies targeting polycystin-related pathways. Current efforts also focus on characterizing its post-translational modifications and ligand-binding properties, leveraging recombinant protein tools for structural biology and drug discovery applications.
×
