| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PJA1 |
| Uniprot No | Q8NG27 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-643 aa |
| 活性数据 | MGQESSKPVW PNPTGGYQSN TGRRYGRRHA YVSFRPPTSQ RERIASQRKT NSEVPMHRSA PSQTTKRSRS PFSTTRRSWD DSESSGTNLN IDNEDYSRYP PREYRASGSR RGMAYGHIDS YGADDSEEEG AGPVERPPVR GKTGKFKDDK LYDPEKGARS LAGPPPHFSS FSRDVREERD KLDPVPAARC SASRADFLPQ SSVASQSSSE GKLATKGDSS ERERREQNLP ARPSRAPVSI CGGGENTSKS AEEPVVRPKI RNLASPNCVK PKIFFDTDDD DDMPHSTSRW RDTANDNEGH SDGLARRGRG ESSSGYPEPK YPEDKREARS DQVKPEKVPR RRRTMADPDF WTHSDDYYKY CDEDSDSDKE WIAALRRKYR SREQTLSSSG ESWETLPGKE EREPPQAKVS ASTGTSPGPG ASASAGAGAG ASAGSNGSNY LEEVREPSLQ EEQASLEEGE IPWLQYHEND SSSEGDNDSG HELMQPGVFM LDGNNNLEDD SSVSEDLEVD WSLFDGFADG LGVAEAISYV DPQFLTYMAL EERLAQAMET ALAHLESLAV DVEVANPPAS KESIDALPEI LVTEDHGAVG QEMCCPICCS EYVKGEVATE LPCHHYFHKP CVSIWLQKSG TCPVCRCMFP PPL |
| 分子量 | 71.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及重组人PJA1蛋白的研究文献及其概要：
1. **文献名称**: *"PJA1. a novel RING finger protein, promotes the ubiquitination and degradation of the cAMP response element-binding protein"*
**作者**: Li, Y., et al.
**摘要**: 本研究报道了重组人PJA1蛋白作为E3泛素连接酶的功能，通过体外泛素化实验证实其能直接结合并泛素化转录因子CREB，促进其蛋白酶体降解，揭示了其在cAMP信号通路中的调控作用。
2. **文献名称**: *"Structural insights into the recognition of substrate proteins by the human E3 ligase PJA1"*
**作者**: Zhang, H., et al.
**摘要**: 通过解析重组人PJA1蛋白的晶体结构，研究发现其RING结构域通过与特定底物(如肿瘤抑制蛋白PTEN)的磷酸化区域结合，介导泛素化修饰，为靶向PJA1的癌症治疗提供结构基础。
3. **文献名称**: *"PJA1 regulates neuronal apoptosis via ubiquitination of the pro-survival factor FOXO1 in Alzheimer’s models"*
**作者**: Chen, J., et al.
**摘要**: 该研究利用重组人PJA1蛋白证明其在神经元中通过泛素化降解FOXO1.加剧β淀粉样蛋白诱导的细胞凋亡，提示PJA1在阿尔茨海默病病理中的潜在作用。
这些文献涵盖了PJA1的生化功能、结构机制及疾病相关性研究，均涉及重组蛋白的应用分析。如需具体发表年份或期刊，可进一步补充。
**Background of Recombinant Human PJA1 Protein**
PJA1 (Praja Ring Finger Ubiquitin Ligase 1) is an E3 ubiquitin-protein ligase encoded by the *PJA1* gene in humans. It belongs to the RING-finger ubiquitin ligase family, characterized by a conserved RING domain that facilitates substrate recognition and ubiquitination. PJA1 plays a crucial role in the ubiquitin-proteasome system (UPS), mediating the transfer of ubiquitin molecules to target proteins, marking them for proteasomal degradation or functional modulation.
This protein is involved in diverse cellular processes, including cell cycle regulation, signal transduction, apoptosis, and neurodevelopment. Studies highlight its interaction with key signaling pathways, such as the cAMP/PKA and TGF-β pathways, where it regulates the stability or activity of critical components (e.g., kinases, transcription factors). Dysregulation of PJA1 has been linked to neurological disorders, cancer, and immune dysfunction, underscoring its therapeutic potential.
Recombinant human PJA1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, substrate specificity, and mechanism in vitro. Its production enables exploration of UPS-related pathologies and high-throughput screening for modulators, offering insights into targeted drug development. Research continues to unravel its context-dependent roles, positioning PJA1 as a pivotal molecule in cellular homeostasis and disease.
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