| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIWIL2 |
| Uniprot No | Q8TC59 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-973 aa |
| 活性数据 | MDPFRPSFRGQSPIHPSQCQAVRMPGCWPQASKPLDPALGRGAPAGRGHVFGKPEEPSTQRGPAQRESVGLVSMFRGLGIETVSKTPLKREMLPSGRGILGRGLSANLVRKDREELSPTFWDPKVLAAGDSKMAETSVGWSRTLGRGSSDASLLPLGRAAGGISREVDKPPCTFSTPSRGPPQLSSPPALPQSPLHSPDRPLVLTVEHKEKELIVKQGSKGTPQSLGLNLVKIQCHNEAVYQYHVTFSPNVECKSMRFGMLKDHQAVTGNVTAFDGSILYLPVKLQQVLELKSQRKTDSAEISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDCVLDVMHAIYQQNKEHFQDECTKLLVGNIVITRYNNRTYRIDDVDWNKTPKDSFTMSDGKEITFLEYYSKNYGITVKEEDQPLLIHRPSERQDNHGMLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLAQQINLSPKQHHSALECLLQRIAKNEAATNELMRWGLRLQKDVHKIEGRVLPMERINLKNTSFITSQELNWVKEVTRDPSILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRMSPPAWVELKDDRIETYVRTIQSTLGAEGKIQMVVCIIMGPRDDLYGAIKKLCCVQSPVPSQVVNVRTIGQPTRLRSVAQKILLQINCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKFYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFENYQPKMVVFVVQKKISTNLYLAAPQNFVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYVCVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHHEPAIQLCENLFFL |
| 分子量 | 133.43 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人PIWIL2蛋白的典型研究文献示例(注：文献及作者为模拟，建议通过学术数据库获取真实资料)：
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1. **文献名称**: *"Recombinant human PIWIL2 protein suppresses cancer cell proliferation by regulating stemness-related pathways"*
**作者**: Liu Y. et al. (2018)
**摘要**: 研究利用重组PIWIL2蛋白处理多种癌细胞系，发现其通过结合piRNA并抑制Wnt/β-catenin通路，显著降低癌细胞的自我更新能力和增殖，提示其在癌症靶向治疗中的潜力。
2. **文献名称**: *"Structural and functional analysis of PIWIL2 reveals its role in epigenetic silencing via piRNA complexes"*
**作者**: Zhang H. et al. (2020)
**摘要**: 通过体外重组表达PIWIL2蛋白，结合X射线晶体学解析其三维结构，发现其PAZ结构域在piRNA结合及染色质重塑中的关键作用，为基因沉默机制提供了新依据。
3. **文献名称**: *"Recombinant PIWIL2 stabilizes germline transcripts and promotes male germ cell development in vitro"*
**作者**: Chen R. et al. (2019)
**摘要**: 研究证明重组PIWIL2蛋白能结合并保护生殖细胞中的mRNA免受降解，促进小鼠精原干细胞的体外分化和成熟，突显其在生殖医学中的应用潜力。
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建议通过 **PubMed** 或 **Google Scholar** 搜索关键词 *"recombinant PIWIL2"* 或 *"PIWIL2 protein function"* 获取最新全文。
**Background of Recombinant Human PIWIL2 Protein**
PIWIL2 (P-element induced wimpy testis-like 2), a member of the Argonaute/PIWI protein family, is primarily expressed in germ cells and plays critical roles in gametogenesis, genome stability, and epigenetic regulation. It binds to PIWI-interacting RNAs (piRNAs), small non-coding RNAs essential for silencing transposable elements during germline development. This interaction safeguards genetic integrity by preventing mutagenic transposon activity, particularly in spermatogenesis and oogenesis.
Recombinant human PIWIL2 protein is engineered using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to enable functional studies. Its structure includes conserved PAZ and PIWI domains, facilitating RNA binding and endonuclease activity. Research highlights its dual role: beyond germ cell function, PIWIL2 is aberrantly expressed in cancers (e.g., gliomas, sarcomas), where it may drive tumorigenesis by promoting stemness, proliferation, and chemoresistance.
Despite its biomedical significance, challenges persist in producing bioactive recombinant PIWIL2 due to structural complexity and post-translational modification requirements. Current applications span dissecting piRNA pathways, exploring transposon silencing mechanisms, and developing therapeutic strategies targeting PIWIL2-overexpressing cancers. Its study bridges reproductive biology, oncology, and regenerative medicine, underscoring its potential as a diagnostic marker or therapeutic target.
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