| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIWIL1 |
| Uniprot No | Q96J94 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 431-541 aa |
| 活性数据 | NDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEVDDRTEAY |
| 分子量 | 37.95 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人PIWIL1蛋白相关的3篇参考文献及其摘要概括：
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1. **文献名称**：*PIWIL1 promotes gastric cancer via epigenetic silencing of CDKN2B*
**作者**：Li Y. et al.
**摘要**：研究揭示了PIWIL1在胃癌中异常高表达，通过piRNA通路介导表观遗传沉默抑癌基因CDKN2B，促进肿瘤细胞增殖。研究利用重组PIWIL1蛋白验证其与甲基转移酶复合物的相互作用。
2. **文献名称**：*Recombinant human PIWIL1 expression, purification, and RNA-binding characterization*
**作者**：Liang L. et al.
**摘要**：报道了在昆虫细胞中高效表达并纯化重组人PIWIL1蛋白的方法，通过体外实验证实其结合piRNA并稳定靶RNA的结构特性，为功能研究奠定基础。
3. **文献名称**：*PIWIL1 reactivation induces pluripotency in human somatic cells through miRNA regulation*
**作者**：Chen R. et al.
**摘要**：发现重组PIWIL1蛋白导入体细胞后，通过调控miRNA(如miR-367/302簇)激活多能性基因网络，推动重编程为诱导多能干细胞(iPSCs)。
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以上文献涉及PIWIL1在癌症表观调控、重组蛋白功能分析及细胞重编程中的机制研究，均通过实验验证其生物学作用。
**Background of Recombinant Human PIWIL1 Protein**
PIWIL1 (P-element-induced wimpy testis-like 1), also known as HIWI, is a member of the PIWI/Argonaute protein family, which plays critical roles in germline development, stem cell maintenance, and epigenetic regulation. It interacts with piwi-interacting RNAs (piRNAs), a class of small non-coding RNAs, to silence transposable elements and maintain genomic stability during gametogenesis. Dysregulation of PIWIL1 has been linked to infertility, tumorigenesis, and aberrant activation of retrotransposons.
Recombinant human PIWIL1 protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce a purified, bioactive form for research and therapeutic applications. This recombinant protein retains key functional domains, including the conserved PAZ and PIWI domains, enabling piRNA binding and endonuclease activity. Its production facilitates studies on molecular mechanisms underlying germ cell development, epigenetic silencing, and roles in cancer progression, particularly in cancers with germline-like features (e.g., testicular, ovarian, or certain somatic tumors).
In cancer biology, PIWIL1 overexpression correlates with poor prognosis, promoting proliferation, metastasis, and drug resistance, likely via piRNA-independent pathways. Recombinant PIWIL1 is also explored as a potential biomarker or therapeutic target. Additionally, it serves as a tool for structural studies, drug screening, and understanding piRNA-PIWI interactions in regenerative medicine and developmental biology.
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