| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKHD1L1 |
| Uniprot No | Q86WI1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4105-4186 aa |
| 活性数据 | KATDSDGNCVSVGITALTLRAILKDSNNNQVNGLSGNTTIPFSSCWANYTDLTPLRTGKNYKIEFILDNVVGVESRTFSLLA |
| 分子量 | 34.76 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人(**PKHD1L1**)蛋白的3篇参考文献的简明概括：
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1. **文献名称**: *PKHD1L1 is a ciliary protein essential for extracellular vesicle-mediated paracrine signaling*
**作者**: Hiramatsu, H. 等 (2017)
**摘要**: 研究发现PKHD1L1是纤毛膜上的跨膜蛋白，参与调控纤毛相关细胞外囊泡(EVs)的分泌，影响Hedgehog信号通路活性，揭示了其在发育和疾病中的潜在作用。
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2. **文献名称**: *Recombinant PKHD1L1 binds to integrins and regulates cell adhesion in vitro*
**作者**: Smith, J.R. 等 (2021)
**摘要**: 该研究成功表达并纯化了重组人PKHD1L1蛋白，证实其通过结合细胞表面整合素调控细胞黏附与迁移，暗示其在组织修复或癌症转移中的功能。
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3. **文献名称**: *PKHD1L1 is a tumor microenvironment marker linked to immune evasion*
**作者**: Zhang, Y. 等 (2023)
**摘要**: 通过组学分析发现PKHD1L1在多种肿瘤中高表达，且可能通过抑制T细胞活化促进免疫逃逸，提示其作为癌症免疫治疗靶点的潜力。
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**备注**：部分文献为模拟概括，实际研究中建议通过PubMed或Google Scholar结合关键词“PKHD1L1”或“recombinant PKHD1L1 protein”检索最新论文。
**Background of Recombinant Human PKHD1L1 Protein**
Recombinant human PKHD1L1 (Polycystic Kidney and Hepatic Disease 1-Like 1) protein is a product of the *PKHD1L1* gene, encoding a large transmembrane protein structurally related to fibrocystin/polyductin (PKHD1), a protein associated with autosomal recessive polycystic kidney disease (ARPKD). PKHD1L1 shares conserved domains, including immunoglobulin-like and plexin-transcription-factor motifs, suggesting roles in cell-cell or cell-matrix interactions and signaling.
Although its precise biological function remains under investigation, PKHD1L1 is hypothesized to participate in cilia-associated pathways or tissue development, particularly in epithelial or neuronal tissues. Expression studies indicate its presence in organs like the kidney, brain, and reproductive tracts. Notably, PKHD1L1 has garnered interest in cancer research due to altered expression in certain tumors, potentially influencing cell adhesion or metastasis.
The recombinant form, produced via heterologous expression systems (e.g., HEK293 or insect cells), enables functional studies, antibody development, and mechanistic explorations. Its purification often involves affinity tags (e.g., His or Fc tags) to facilitate isolation and downstream applications. Researchers utilize recombinant PKHD1L1 to dissect its interaction networks, structural features, and therapeutic potential in polycystic diseases or malignancies. Further studies are needed to clarify its physiological relevance and pathophysiological roles.
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