| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKMYT1 |
| Uniprot No | Q99640 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-499 aa |
| 活性数据 | MLERPPALAM PMPTEGTPPP LSGTPIPVPA YFRHAEPGFS LKRPRGLSRS LPPPPPAKGS IPISRLFPPR TPGWHQLQPR RVSFRGEASE TLQSPGYDPS RPESFFQQSF QRLSRLGHGS YGEVFKVRSK EDGRLYAVKR SMSPFRGPKD RARKLAEVGS HEKVGQHPCC VRLEQAWEEG GILYLQTELC GPSLQQHCEA WGASLPEAQV WGYLRDTLLA LAHLHSQGLV HLDVKPANIF LGPRGRCKLG DFGLLVELGT AGAGEVQEGD PRYMAPELLQ GSYGTAADVF SLGLTILEVA CNMELPHGGE GWQQLRQGYL PPEFTAGLSS ELRSVLVMML EPDPKLRATA EALLALPVLR QPRAWGVLWC MAAEALSRGW ALWQALLALL CWLWHGLAHP ASWLQPLGPP ATPPGSPPCS LLLDSSLSSN WDDDSLGPSL SPEAVLARTV GSTSTPRSRC TPRDALDLSD INSEPPRGSF PSFEPRNLLS LFEDTLDPT |
| 分子量 | 54.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人PKMYT1蛋白的参考文献示例(虚构内容，供参考)：
1. **"Characterization of Recombinant Human PKMYT1 Kinase Activity in Cell Cycle Regulation"**
- **作者**: Zhang et al.
- **摘要**: 研究通过大肠杆菌表达系统成功纯化重组人PKMYT1蛋白，证实其通过磷酸化CDK1-Tyr15调控G2/M期转换，并发现其活性受PLK1激酶调控。
2. **"PKMYT1 as a Therapeutic Target in BRCA-Deficient Cancers"**
- **作者**: Brown & Thompson
- **摘要**: 揭示PKMYT1在BRCA突变肿瘤中通过合成致死机制成为潜在靶点，利用重组PKMYT1蛋白筛选小分子抑制剂，并验证其在卵巢癌细胞中的抗增殖效果。
3. **"Structural Insights into PKMYT1 Inhibitor Binding by Cryo-EM"**
- **作者**: Lee et al.
- **摘要**: 通过冷冻电镜解析重组人PKMYT1蛋白与抑制剂复合物的三维结构，阐明其ATP结合口袋特征，为优化靶向药物设计提供依据。
4. **"High-Yield Production of Active PKMYT1 in Insect Cells for Functional Studies"**
- **作者**: Müller et al.
- **摘要**: 开发基于杆状病毒昆虫细胞系统的重组PKMYT1表达方法，实现高活性蛋白量产，并用于体外激酶活性检测及抗体开发。
(注：上述文献为模拟示例，实际研究需查阅真实数据库如PubMed或Web of Science。)
**Background of Recombinant Human PKMYT1 Protein**
Recombinant human PKMYT1 (Protein Kinase Membrane-Associated Tyrosine/Threonine 1) is a cell cycle-regulated kinase belonging to the Ser/Thr protein kinase family. It is structurally related to WEE1 kinases and plays a critical role in regulating the G2/M phase transition by inhibiting cyclin-dependent kinase 1 (CDK1), thereby preventing premature entry into mitosis. PKMYT1 specifically phosphorylates CDK1 at Thr14. contributing to checkpoint control that ensures genomic stability during DNA replication.
Produced via recombinant DNA technology in expression systems like *E. coli* or mammalian cells, recombinant PKMYT1 retains post-translational modifications and functional activity, enabling its use in biochemical assays and drug discovery. Its dysregulation has been linked to cancer, where overexpression may promote unchecked cell proliferation. This makes PKMYT1 a potential therapeutic target, particularly in cancers resistant to conventional therapies targeting related kinases like WEE1.
Current research focuses on elucidating PKMYT1’s structural dynamics, substrate interactions, and role in cancer pathways. Inhibitors under development aim to disrupt its kinase activity, offering novel strategies for precision oncology. Overall, recombinant PKMYT1 serves as a vital tool for dissecting cell cycle mechanisms and advancing targeted anticancer therapies.
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