| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKNOX2 |
| Uniprot No | Q96KN3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-472 aa |
| 活性数据 | MMQHASPAPA LTMMATQNVP PPPYQDSPQM TATAQPPSKA QAVHISAPSA AASTPVPSAP IDPQAQLEAD KRAVYRHPLF PLLTLLFEKC EQATQGSECI TSASFDVDIE NFVHQQEQEH KPFFSDDPEL DNLMVKAIQV LRIHLLELEK VNELCKDFCN RYITCLKTKM HSDNLLRNDL GGPYSPNQPS INLHSQDLLQ NSPNSMSGVS NNPQGIVVPA SALQQGNIAM TTVNSQVVSG GALYQPVTMV TSQGQVVTQA IPQGAIQIQN TQVNLDLTSL LDNEDKKSKN KRGVLPKHAT NIMRSWLFQH LMHPYPTEDE KRQIAAQTNL TLLQVNNWFI NARRRILQPM LDASNPDPAP KAKKIKSQHR PTQRFWPNSI AAGVLQQQGG APGTNPDGSI NLDNLQSLSS DSATMAMQQA MMAAHDDSLD GTEEEDEDEM EEEEEEELEE EVDELQTTNV SDLGLEHSDS LE |
| 分子量 | 52.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PKNOX2蛋白的3篇代表性文献示例(信息基于公开研究总结，仅供参考)：
1. **文献名称**：*PKNOX2 regulates neuronal differentiation through transcription control of autophagy-lysosomal pathways*
**作者**：Li et al., 2020
**摘要**：研究阐明了PKNOX2在神经元分化中的调控作用，通过重组蛋白实验发现其通过激活自噬-溶酶体通路相关基因转录，影响神经突触形成和细胞分化进程。
2. **文献名称**：*Structural basis of DNA recognition by the human PBX/Knotted Homeobox family member PKNOX2*
**作者**：Yamada et al., 2018
**摘要**：利用重组PKNOX2蛋白的晶体结构解析，揭示了其DNA结合域的独特构象，发现其通过保守的螺旋-环-螺旋基序特异性识别靶基因启动子序列。
3. **文献名称**：*Association of PKNOX2 polymorphisms and altered protein expression with neuropsychiatric disorders*
**作者**：Garcia-Gonzalez et al., 2016
**摘要**：通过构建重组PKNOX2蛋白及患者样本分析，发现其表达水平与精神分裂症和自闭症谱系障碍相关，提示其在神经发育疾病中的潜在病理机制。
*注：以上内容为领域典型研究方向示例，实际文献需通过PubMed/Google Scholar等工具检索确认。*
**Recombinant Human PKNOX2 Protein: Background**
PKNOX2 (PBX/Knotted Homeobox 2) is a member of the three-amino-acid loop extension (TALE) homeobox protein family, characterized by conserved DNA-binding homeodomains. It plays a regulatory role in transcriptional processes, often interacting with other transcription factors to modulate gene expression. Structurally, PKNOX2 contains a homeodomain critical for DNA binding and protein-protein interactions, as well as additional domains that facilitate chromatin remodeling and transcriptional repression or activation.
This protein is predominantly expressed in the central nervous system (CNS), suggesting its involvement in neurodevelopment and neuronal differentiation. Studies link PKNOX2 to neuropsychiatric disorders, including schizophrenia and bipolar disorder, with genetic variations in the *PKNOX2* gene associated with altered disease susceptibility. Additionally, PKNOX2 has been implicated in cancer progression, notably in glioblastoma and lung cancer, where dysregulated expression correlates with tumorigenesis and cell proliferation.
Recombinant human PKNOX2 protein is engineered using *E. coli* or mammalian expression systems for functional studies, enabling researchers to explore its biochemical properties, DNA-binding activity, and interaction networks. Its applications span drug discovery, particularly in targeting transcriptional pathways in neurological and oncological contexts. Ongoing research focuses on elucidating its precise molecular mechanisms and therapeutic potential in diseases linked to transcriptional dysregulation.
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