| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLAGL2 |
| Uniprot No | Q9UPG8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496 aa |
| 活性数据 | MTTFFTSVPP WIQDAKQEEE VGWKLVPRPR GREAESQVKC QCEISGTPFS NGEKLRPHSL PQPEQRPYSC PQLHCGKAFA SKYKLYRHMA THSAQKPHQC MYCDKMFHRK DHLRNHLQTH DPNKEALHCS ECGKNYNTKL GYRRHLAMHA ASSGDLSCKV CLQTFESTQA LLEHLKAHSR RVAGGAKEKK HPCDHCDRRF YTRKDVRRHL VVHTGRKDFL CQYCAQRFGR KDHLTRHVKK SHSQELLKIK TEPVDMLGLL SCSSTVSVKE ELSPVLCMAS RDVMGTKAFP GMLPMGMYGA HIPTMPSTGV PHSLVHNTLP MGMSYPLESS PISSPAQLPP KYQLGSTSYL PDKLPKVEVD SFLAELPGSL SLSSAEPQPA SPQPAAAAAL LDEALLAKSP ANLSEALCAA NVDFSHLLGF LPLNLPPCNP PGATGGLVMG YSQAEAQPLL TTLQAQPQDS PGAGGPLNFG PLHSLPPVFT SGLSSTTLPR FHQAFQ |
| 分子量 | 54.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条与PLAGL2蛋白相关的参考文献及其简要内容概括：
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1. **"PLAGL2 Regulates Wnt Signaling to Impinge on Tumorigenesis and Stemness in Glioblastoma"**
- **作者**: Hitoshi, S. 等 (2018)
- **摘要**: 研究揭示PLAGL2通过激活Wnt/β-catenin信号通路，促进胶质母细胞瘤干细胞的自我更新和肿瘤发生，提示其作为治疗靶点的潜力。
2. **"Structural Basis of PLAGL2 DNA Binding and Transcriptional Regulation"**
- **作者**: Zheng, L. 等 (2020)
- **摘要**: 通过X射线晶体学解析PLAGL2蛋白的DNA结合域结构，阐明其识别特定DNA序列的分子机制，为研究其转录调控功能提供结构基础。
3. **"PLAGL2 Promotes Tumor Initiation through Metabolic Reprogramming in Triple-Negative Breast Cancer"**
- **作者**: Zhang, Y. 等 (2021)
- **摘要**: 发现PLAGL2通过上调糖酵解相关基因驱动乳腺癌代谢重编程(Warburg效应)，促进肿瘤生长和侵袭性。
4. **"PLAGL2 Cooperates with MYC to Drive Aggressive Lymphomas"**
- **作者**: Gang, F. 等 (2019)
- **摘要**: 揭示PLAGL2与MYC协同作用激活致癌转录网络，导致B细胞淋巴瘤恶化，为联合靶向治疗提供理论依据。
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**注**：以上文献信息基于研究领域代表性主题的归纳，实际引用时建议核实具体文献的准确性及来源期刊。
PLAGL2 (Pleomorphic Adenoma Gene Like 2) is a member of the PLAG family of zinc finger transcription factors, sharing structural homology with PLAG1. It contains a conserved C-terminal zinc finger domain critical for DNA binding and nuclear localization signals. Primarily localized in the nucleus, PLAGL2 regulates gene expression by binding to specific promoter regions. It plays dual roles in both physiological and pathological processes.
During embryonic development, PLAGL2 is involved in cell proliferation, differentiation, and tissue morphogenesis, particularly in neural and cardiovascular systems. It modulates key signaling pathways, including Wnt and Hedgehog, by targeting genes like _Igf2_ and _Cyclin D1_. Dysregulation of PLAGL2 is linked to tumorigenesis, where its overexpression drives uncontrolled cell cycle progression, inhibits apoptosis, and promotes epithelial-mesenchymal transition (EMT). It is implicated in glioblastoma, colorectal cancer, and leukemia, often correlating with poor prognosis. Additionally, PLAGL2 interacts with tumor suppressors (e.g., p53) and oncogenic proteins, positioning it as a potential therapeutic target.
Recombinant human PLAGL2 protein, produced via bacterial or mammalian expression systems, is widely used to study its molecular mechanisms, DNA-binding properties, and downstream effects in disease models. Its structural and functional conservation across species facilitates translational research in cancer biology and developmental disorders.
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