| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLEKHC1 |
| Uniprot No | Q96AC1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-680 aa |
| 活性数据 | MALDGIRMPD GCYADGTWEL SVHVTDLNRD VTLRVTGEVH IGGVMLKLVE KLDVKKDWSD HALWWEKKRT WLLKTHWTLD KYGIQADAKL QFTPQHKLLR LQLPNMKYVK VKVNFSDRVF KAVSDICKTF NIRHPEELSL LKKPRDPTKK KKKKLDDQSE DEALELEGPL ITPGSGSIYS SPGLYSKTMT PTYDAHDGSP LSPTSAWFGD SALSEGNPGI LAVSQPITSP EILAKMFKPQ ALLDKAKINQ GWLDSSRSLM EQDVKENEAL LLRFKYYSFF DLNPKYDAIR INQLYEQAKW AILLEEIECT EEEMMMFAAL QYHINKLSIM TSENHLNNSD KEVDEVDAAL SDLEITLEGG KTSTILGDIT SIPELADYIK VFKPKKLTLK GYKQYWCTFK DTSISCYKSK EESSGTPAHQ MNLRGCEVTP DVNISGQKFN IKLLIPVAEG MNEIWLRCDN EKQYAHWMAA CRLASKGKTM ADSSYNLEVQ NILSFLKMQH LNPDPQLIPE QITTDITPEC LVSPRYLKKY KNKQITARIL EAHQNVAQMS LIEAKMRFIQ AWQSLPEFGI THFIARFQGG KKEELIGIAY NRLIRMDAST GDAIKTWRFS NMKQWNVNWE IKMVTVEFAD EVRLSFICTE VDCKVVHEFI GGYIFLSTRA KDQNESLDEE MFYKLTSGWV |
| 分子量 | 77.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PLEKHC1蛋白的模拟参考文献示例(注：以下内容为假设性示例，非真实存在的文献)：
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1. **文献名称**: *PLEKHC1 regulates cytoskeletal dynamics through interaction with PI3K in cancer cell migration*
**作者**: Smith A, et al.
**摘要**: 本研究报道了PLEKHC1通过其pleckstrin同源结构域与PI3K信号通路相互作用，调控肌动蛋白重组，促进肿瘤细胞的迁移和侵袭。重组PLEKHC1蛋白的体外功能实验验证了其在细胞骨架重塑中的关键作用。
2. **文献名称**: *Structural insights into the membrane association mechanism of human PLEKHC1*
**作者**: Zhang Y, et al.
**摘要**: 通过X射线晶体学解析了PLEKHC1蛋白的结构，揭示其PH结构域与磷脂膜的相互作用机制，并发现其C端螺旋对细胞膜定位的调控，为理解其在细胞内运输中的功能提供结构基础。
3. **文献名称**: *PLEKHC1 deficiency disrupts autophagy and promotes neurodegeneration in a Drosophila model*
**作者**: Lee J, et al.
**摘要**: 利用果蝇模型研究PLEKHC1缺失对自噬的影响，发现PLEKHC1通过调控自噬体形成维持神经元稳态，其重组蛋白回补实验表明功能保守性，提示与神经退行性疾病潜在关联。
4. **文献名称**: *High-throughput screening identifies PLEKHC1 as a novel biomarker in colorectal cancer*
**作者**: Wang H, et al.
**摘要**: 通过蛋白质组学分析发现PLEKHC1在结直肠癌组织中的异常高表达，重组蛋白的体外实验证实其通过激活Wnt/β-catenin通路促进肿瘤增殖，提示其作为治疗靶点的潜力。
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*注：以上文献及内容均为示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索最新成果。*
PLEKHc1. also known as Pleckstrin Homology and Coiled-Coil Domain-Containing Protein 1. is a multifunctional adapter protein encoded by the human *PLEKHC1* gene. It belongs to the pleckstrin homology (PH) domain-containing protein family, which typically participates in intracellular signaling, membrane trafficking, and cytoskeletal organization. Structurally, PLEKHC1 contains an N-terminal PH domain capable of binding phosphoinositides or other signaling molecules, and a C-terminal coiled-coil domain that facilitates protein-protein interactions. These features enable it to act as a scaffold or mediator in cellular processes.
Studies suggest that PLEKHC1 plays roles in regulating vesicle transport, endocytosis, and actin cytoskeleton dynamics. It has been implicated in interactions with motor proteins like myosin VI, potentially influencing Golgi-to-plasma membrane trafficking. Emerging research links PLEKHC1 to disease pathways, including cancer progression and neurological disorders. For instance, altered PLEKHC1 expression has been observed in certain tumors, where it may modulate cell migration or apoptosis. Its involvement in maintaining neuronal integrity has also drawn attention in neurodegeneration studies.
Recombinant human PLEKHC1 protein is commonly produced in *E. coli* or mammalian expression systems for functional studies, structural analysis, or screening therapeutic targets. Ongoing research aims to clarify its precise mechanisms and therapeutic potential across diseases.
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