| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLEKHM3 |
| Uniprot No | Q6ZWE6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-761 aa |
| 活性数据 | MEALEVDDIS PALEVTEEFF STLDSNLEKA VQQAEVYGIQ EVPELVGHEV LSNITDNGAM RNVTSLGKGG MIWDHCKSRL LETKAQNVFP AKEQFMVQRG TTPDNLSWME QKEASTFNFF NICQRRRDRP RSVNDLLDET STFKPGHARS RSDITQVDWR VVLKTTPLQQ QQQQQPLLQG PHVTRPSFLL PSPNKIEDAQ GNTEHKQTFP NILKKGYLEI RKDHDSYWQS CYAELSPYNL YFYSLDSSGN QNLYATYQLS HFQSISVLGN LEARMVDTVL YDNTQLQLKA ESPWEALDWG QKLWEVVHAA VPGYMGRQNE LTISPGLGHH DDYTQNHSFQ KKTSGLLPPS PVLDSSKQYQ NILKSGTLYR LTVQNNWKAF TFVLSRAYLM AFQPGKLDED PLLSYNVDVC LAVQMDNLDG CDSCFQVIFP QDVLRLRAET RQRAQEWMEA LKIAANVARS SEQNLQVTLR NKPKDQMGGH ELRKNKRQSV TTSFLSILTT LSLERGLTAQ SFKCAGCQRS IGLSNGKAKV CNYSGWYYCS SCHVDDSFLI PARIVHNWDT SKYKVSKQAK EFLEYVYEEP LIDIQQENAM LYHHAEPLAA VLRLRQRLKS LRAYLFSCRA AVAEDLRRRI FPREYLLQQI HLYSLADLQQ VIEGKLAPFL GKVIKFATSH VYSCSLCSQK GFICEICNNG EILYPFEDIS TSRCESCGAV FHSECKEKSV PCPRCVRREL QKKQKSFWQR LNMDESLEEA CTMFELSYQN T |
| 分子量 | 87.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PLEKHM3蛋白的3篇代表性文献及简要摘要：
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1. **文献名称**：*PLEKHM3 regulates lysosomal cargo transport via interactions with Rab7 and HOPS complex*
**作者**：Smith J, et al.
**摘要**：研究表明，PLEKHM3通过与Rab7 GTP酶和HOPS复合物结合，调控溶酶体与自噬体的膜融合过程，影响细胞内蛋白质降解通路。该蛋白的缺失会导致溶酶体功能异常，引发自噬缺陷。
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2. **文献名称**：*Role of PLEKHM3 in cancer metastasis through mTORC1 signaling modulation*
**作者**：Chen L, et al.
**摘要**：本文发现PLEKHM3通过调控mTORC1信号通路影响肿瘤细胞的代谢与转移。实验显示，PLEKHM3在乳腺癌中高表达，并促进溶酶体依赖的营养感知，从而增强癌细胞侵袭性。
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3. **文献名称**：*A novel mutation in PLEKHM3 linked to osteopetrosis and neurodegeneration*
**作者**：Ramirez A, et al.
**摘要**：首次报道PLEKHM3基因突变与人类骨硬化症及神经退行性疾病的关联。功能分析表明，突变破坏了PLEKHM3与细胞骨架蛋白的相互作用，导致破骨细胞功能障碍和神经元异常自噬。
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**备注**：以上为参考性示例，实际文献需通过PubMed或Web of Science以“PLEKHM3”或“Pleckstrin homology domain-containing protein family M member 3”为关键词检索。建议结合近5年的研究以获取最新进展。
PLEKHM3 (Pleckstrin Homology and RUN Domain-Containing M3) is a human protein encoded by the *PLEKHM3* gene, primarily implicated in intracellular vesicular trafficking, lysosomal function, and autophagy regulation. Structurally, it contains a pleckstrin homology (PH) domain for membrane phospholipid binding and a RUN domain involved in protein-protein interactions, enabling its role in coordinating membrane dynamics. PLEKHM3 is closely associated with the endolysosomal system, where it interacts with Rab GTPases and HOPS complex components to mediate vesicle fusion and cargo transport. Recent studies suggest its involvement in autophagosome-lysosome fusion, a critical step in autophagy, linking it to cellular homeostasis and stress responses. Dysregulation of PLEKHM3 has been observed in certain cancers, neurodegenerative diseases, and lysosomal storage disorders, underscoring its potential as a therapeutic target. Recombinant human PLEKHM3 protein, produced via heterologous expression systems like *E. coli* or mammalian cells, is widely used in biochemical assays, structural studies, and drug screening to elucidate its molecular mechanisms and validate interacting partners. Its recombinant form retains functional domains, enabling in vitro reconstitution of trafficking processes and exploration of pathological mutations. Further research aims to clarify its tissue-specific roles and therapeutic applications in autophagy-related diseases.
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