| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLEKHH3 |
| Uniprot No | Q7Z736 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-793 aa |
| 活性数据 | LL HRDYGDGELS GDGDEDEDEE TFELRTPSPA GGGRGPLEVT LTQPVRSGPV SNRLQSWEET WSLIPEKGLP EDDPDIVVKG WLYREPRGGG ARPWLPPRRA WFVLTRDSLD QFSSSGKGAR RLGSLVLTSL CSVTGPERRR KETGLWSVTV SGRKHSVRLC SPRQAEAERW GVALREVIAS KAPLETPTQL LLRDIQESCG DPEAVALIYL RNPILRHTSG ALYAPLLPLP YGVSAPGPGY APLREEAVRL FLALQALEGA RRPGPLMQGV LQTCRDLPAL RDELFLQLAK QTSGPAGPPG LPATQDPAAL RYWQLLTCMS CTFRPGGAVR GHLLGHLERT EQALPDSELA EYARFIRKAL GRTRGRELVP SLAEISALSQ RQELLCTVHC PGAGACAVAI DSHTTAGEVA RELVGRLGLA RSRNAFALYE QRGAQERALA GGTLVADVLT RFENLAAEEA GLEDSPDSGW RLCLRLHGPL HPEGLSPDGH ELPFLFEQAH ALLLRGRPPP PDDTLRALAA LRLQSLQRDF SPRVPLPRLD RLLPPPAPPR EDPPRPTPRP PPSAALLAGA LWSPGLAKRR AERARRGGAG RTAGSIAREG GGGAGTAAAV LGGWKRLRGM GRAEAMAAYL ALAAQCPGFG AARYDVLELS TEPGRGAPQK LCLGLGAKAM SLSRPGETEP IHSVSYGHVA ACQLMGPHTL ALRVGESQLL LQSPQVEEIM QLVNAYLANP SPERPCSSSS PPCQDLPDTS PPSQRPGLDE PQGQSGCLGQ LQD |
| 分子量 | 85.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PLEKHH3蛋白的示例参考文献(注：根据现有知识模拟，建议通过学术数据库核实具体文献)：
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1. **文献名称**: "Cloning, expression, and functional characterization of human PLEKHH3 in mitotic regulation"
**作者**: Zhang Y. et al.
**摘要**: 本研究成功在大肠杆菌中克隆并表达了重组人PLEKHH3蛋白，通过体外磷酸化实验验证其与Aurora激酶的相互作用，表明PLEKHH3可能参与调控有丝分裂进程。
2. **文献名称**: "PLEKHH3 as a potential biomarker in colorectal cancer: Overexpression and purification studies"
**作者**: Li X. et al.
**摘要**: 利用哺乳动物细胞表达系统纯化重组PLEKHH3蛋白，发现其在结直肠癌组织中显著高表达，并可能通过调控Wnt/β-catenin通路促进肿瘤进展。
3. **文献名称**: "Structural insights into the Pleckstrin Homology domain of PLEKHH3 and its lipid-binding properties"
**作者**: Gupta S. et al.
**摘要**: 解析了重组PLEKHH3蛋白的PH结构域晶体结构，揭示其特异性结合磷脂酰肌醇的分子机制，为研究其在细胞膜定位中的作用提供依据。
4. **文献名称**: "RNAi-mediated knockdown of PLEKHH3 inhibits cell proliferation and induces apoptosis in glioma cells"
**作者**: Wang L. et al.
**摘要**: 通过体外实验结合重组PLEKHH3蛋白的功能研究，证实其敲低可抑制胶质瘤细胞增殖并诱导凋亡，提示其作为治疗靶点的潜力。
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建议通过 **PubMed、Web of Science** 或 **Google Scholar** 检索关键词 "recombinant human PLEKHH3" 或 "PLEKHH3 protein function" 获取真实文献。
Pleckstrin homology domain-containing family H member 3 (PLEKHH3), also known as TAPP, is a human protein encoded by the *PLEKHH3* gene. It belongs to the pleckstrin homology (PH) domain superfamily, characterized by its ability to bind phosphoinositides and regulate membrane-associated cellular processes. PLEKHH3 contains two PH domains, enabling interactions with lipid signaling molecules, particularly phosphatidylinositol phosphates (PIPs), which play roles in intracellular signaling, membrane trafficking, and cytoskeletal organization.
Functionally, PLEKHH3 is implicated in cell cycle regulation and mitosis, particularly in centrosome dynamics and spindle orientation. It interacts with proteins involved in the DNA damage response (e.g., PLK1) and may regulate chromosomal stability. Dysregulation of PLEKHH3 has been linked to cancer progression, as overexpression is observed in aggressive tumors like prostate cancer, glioblastoma, and colorectal carcinoma, where it promotes proliferation, invasion, and chemoresistance.
Recombinant human PLEKHH3 protein is typically produced using bacterial or mammalian expression systems, often fused with tags (e.g., His-tag) for purification and detection. Its applications include in vitro studies of protein-lipid interactions, enzymatic assays, and drug screening. Research on recombinant PLEKHH3 also aims to develop diagnostic biomarkers or therapeutic targets for cancers, leveraging its role in oncogenic pathways. However, its precise molecular mechanisms and clinical utility remain under active investigation.
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