| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLEKHN1 |
| Uniprot No | Q494U1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-663 aa |
| 活性数据 | MGNSHCVPQA PRRLRASFSR KPSLKGNRED SARMSAGLPG PEAARSGDAA ANKLFHYIPG TDILDLENQR ENLEQPFLSV FKKGRRRVPV RNLGKVVHYA KVQLRFQHSQ DVSDCYLELF PAHLYFQAHG SEGLTFQGLL PLTELSVCPL EGSREHAFQI TGVWDASRAP RGTPDPGLGE GPALWLRSTC VYVCALSALP AGPLPAPLLV LCPSRAELDR WLYHLEKQTA LLGGPRRCHS APPQGSCGDE LPWTLQRRLT RLRTASGHEP GGSAVCASRV KLQHLPAQEQ WDRLLVLYPT SLAIFSEELD GLCFKGELPL RAVHINLEEK EKQIRSFLIE GPLINTIRVV CASYEDYGHW LLCLRAVTHR EGAPPLPGAE SFPGSQVMGS GRGSLSSGGQ TSWDSGCLAP PSTRTSHSLP ESSVPSTVGC SSQHTPDQAN SDRASIGRRR TELRRSGSSR SPGSKARAEG RGPVTPLHLD LTQLHRLSLE SSPDAPDHTS ETSHSPLYAD PYTPPATSHR RVTDVRGLEE FLSAMQSARG PTPSSPLPSV PVSVPASDPR SCSSGPAGPY LLSKKGALQS RAAQRHRGSA KDGGPQPPDA PQLVSSAREG SPEPWLPLTD GRSPRRSRDP GYDHLWDETL SSSHQKCPQL GGPEASGGLV QWI |
| 分子量 | 66.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PLEKHN1蛋白的参考文献示例(注：因该蛋白研究较少，以下内容为假设性示例，建议通过学术数据库进一步验证)：
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1. **文献名称**: *"Expression and purification of recombinant human PLEKHN1 in Escherichia coli for functional studies"*
**作者**: Chen L, et al.
**摘要**: 报道了利用大肠杆菌系统高效表达并纯化重组人PLEKHN1蛋白的方法，验证了其与细胞骨架蛋白的体外相互作用，为后续功能研究奠定基础。
2. **文献名称**: *"PLEKHN1 regulates NF-κB signaling in inflammatory responses"*
**作者**: Kim S, Park JH.
**摘要**: 通过基因敲除实验发现，重组PLEKHN1蛋白通过调控NF-κB信号通路参与巨噬细胞的炎症反应，提示其在免疫疾病中的潜在作用。
3. **文献名称**: *"Structural insights into PLEKHN1's role in vesicle trafficking"*
**作者**: Watanabe R, et al.
**摘要**: 利用X射线晶体学解析了重组PLEKHN1蛋白的C端结构域，揭示其与细胞内囊泡运输相关蛋白的结合机制，提出其在胞内运输中的调控功能。
4. **文献名称**: *"PLEKHN1 knockdown suppresses tumor cell migration in breast cancer models"*
**作者**: Zhang Y, et al.
**摘要**: 发现重组PLEKHN1蛋白的过表达促进乳腺癌细胞迁移，其缺失通过抑制RhoA通路降低侵袭能力，提示其作为癌症治疗靶点的可能性。
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**注意**：以上文献为示例性质，实际研究中PLEKHN1可能与其他蛋白(如PLEKHM1)存在名称混淆，建议通过基因数据库(如NCBI Gene)确认靶点信息后，结合关键词在PubMed或Web of Science检索最新文献。
Pleckstrin homology domain-containing family member O1 (PLEKHO1. also known as PLEKHN1), is a ubiquitously expressed human protein encoded by the *PLEKHO1* gene located on chromosome 1q21.3. It belongs to the pleckstrin homology (PH) domain superfamily, characterized by a conserved PH domain that facilitates interactions with membrane phospholipids or protein partners. PLEKHO1 additionally contains a C-terminal coiled-coil region, suggesting roles in protein-protein interactions or subcellular localization.
Though less studied than other PH domain proteins, PLEKHO1 is implicated in diverse cellular processes. It regulates the ubiquitin-proteasome system by interacting with deubiquitinating enzymes (DUBs) like USP8. influencing protein stability and turnover. PLEKHO1 also modulates autophagy, apoptosis, and inflammatory signaling pathways (e.g., NF-κB), with implications in cancer progression, neurodegenerative disorders, and immune responses. Dysregulation of PLEKHO1 has been observed in malignancies, where it may act as a tumor suppressor or promoter depending on context.
Recombinant human PLEKHO1 protein is typically produced in *E. coli* or mammalian expression systems for functional studies. Its applications include investigating molecular mechanisms in disease models, screening therapeutic agents targeting autophagy or proteostasis, and exploring interactions within signaling networks. Despite emerging roles, PLEKHO1’s full functional spectrum and clinical relevance remain under investigation, highlighting its potential as a research and therapeutic target.
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