| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POF1B |
| Uniprot No | Q8WVV4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-589 aa |
| 活性数据 | MSSSYWSETS SSSCGTQQLP EVLQCQPQHY HCYHQSSQAQ QPPEKNVVYE RVRTYSGPMN KVVQALDPFN SREVLSPLKT TSSYQNLVWS DHSQELHSPT LKISTCAPST LHITQNTEQE LHSPTVKLTT YPQTTIRKYV VQNPEQEPLS QFLRGSHFFP GNNVIYEKTI RKVEKLNTDQ GCHPQAQCHH HIIQQPQVIH SAHWQQPDSS QQIQAITGNN PISTHIGNEL CHSGSSQICE QVIIQDDGPE KLDPRYFGEL LADLSRKNTD LYHCLLEHLQ RIGGSKQDFE STDESEDIES LIPKGLSEFT KQQIRYILQM RGMSDKSLRL VLSTFSNIRE ELGHLQNDMT SLENDKMRLE KDLSFKDTQL KEYEELLASV RANNHQQQQG LQDSSSKCQA LEENNLSLRH TLSDMEYRLK ELEYCKRNLE QENQNLRMQV SETCTGPMLQ AKMDEIGNHY TEMVKNLRME KDREICRLRS QLNQYHKDVS KREGSCSDFQ FKLHELTSLL EEKDSLIKRQ SEELSKLRQE IYSSHNQPST GGRTTITTKK YRTQYPILGL LYDDYEYIPP GSETQTIVIE KTEDKYTCP |
| 分子量 | 68.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POF1B蛋白的3篇虚构参考文献示例，供参考：
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1. **标题**：*Recombinant Human POF1B Expression in E. coli and Its Role in Cell Adhesion*
**作者**：Zhang L, et al.
**摘要**：本研究在大肠杆菌中成功表达了可溶性的重组POF1B蛋白，并通过亲和层析纯化。实验表明POF1B通过调控β-catenin磷酸化增强上皮细胞间粘附，为研究其在肿瘤转移中的作用提供了依据。
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2. **标题**：*Structural Analysis of POF1B Reveals O-Fucosylation Catalytic Mechanism*
**作者**：Martinez R, et al.
**摘要**：通过X射线晶体学解析了重组POF1B蛋白的三维结构，鉴定了其底物结合域及关键活性位点(Asp189和His220)，揭示了其在Notch信号通路中催化O-岩藻糖基化的分子机制。
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3. **标题**：*POF1B Knockdown Alters Cytoskeleton Dynamics via Rho-GTPase Pathway*
**作者**：Chen X, et al.
**摘要**：利用siRNA沉默POF1B基因后，细胞迁移能力显著下降。机制研究表明，POF1B通过调节RhoA/ROCK信号通路影响肌动蛋白重组，提示其可能作为癌症治疗的潜在靶点。
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注：以上文献为模拟内容，实际研究中请以权威数据库(如PubMed)检索结果为准。
POF1B (Premature Ovarian Failure 1B) protein, also known as PDZK7 or C9orf3. is a cytoskeletal regulator encoded by the *POF1B* gene located on chromosome Xq21.1. Initially identified through its association with premature ovarian insufficiency (POI), POF1B plays a critical role in maintaining cell polarity, epithelial integrity, and actin cytoskeleton organization. Structurally, it contains a PDZ domain, which facilitates protein-protein interactions, and a coiled-coil region involved in oligomerization.
POF1B interacts with components of cell adhesion complexes, such as E-cadherin and β-catenin, to stabilize apical-basal polarity in epithelial tissues. Its function extends beyond reproductive health; studies link POF1B to cancer progression, particularly in modulating metastasis through cytoskeletal dynamics in epithelial-derived tumors. Knockout models reveal its necessity in female fertility, as POF1B-deficient mice exhibit ovarian follicle depletion and sterility.
Recombinant human POF1B protein is produced via expression systems (e.g., *E. coli* or mammalian cells) for functional studies, enabling exploration of its molecular mechanisms in disease contexts. Research applications include investigating its role in signaling pathways (e.g., TGF-β) and validating interactions with binding partners using techniques like pull-down assays. Dysregulation of POF1B is implicated in epithelial barrier dysfunction, infertility, and oncogenesis, highlighting its therapeutic potential. Current studies focus on leveraging recombinant POF1B to develop biomarkers for ovarian reserve assessment or targeted therapies for cancers exploiting cytoskeletal vulnerabilities.
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