| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PNO1 |
| Uniprot No | Q9NRX1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-252 aa |
| 活性数据 | MESEMETQSARAEEGFTQVTRKGGRRAKKRQAEQLSAAGEGGDAGRMDTEEARPAKRPVFPPLCGDGLLSGKEETRKIPVPANRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNVEIRTCKETKDVSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEITDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADVKVHILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSADRF |
| 分子量 | 34.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于假设的示例文献(仅供参考，具体文献请查询数据库)：
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**1. "Recombinant Expression and Functional Characterization of Human PNO1 in Ribosome Biogenesis"**
*作者：Zhang L, et al.*
摘要：研究通过大肠杆菌系统重组表达人PNO1蛋白，并验证其在核糖体RNA加工中的功能，揭示其与NOB1的相互作用对前体rRNA切割的调控作用。
**2. "Structural Insights into the Role of PNO1 in Ribosome Assembly"**
*作者：Müller R, et al.*
摘要：利用昆虫细胞系统表达重组人PNO1.通过X射线晶体学解析其三维结构，阐明其参与核糖体亚基成熟的分子机制。
**3. "Purification and Biochemical Analysis of Recombinant Human PNO1 for Drug Screening"**
*作者：Chen Y, et al.*
摘要：优化重组人PNO1蛋白的真核表达及亲和纯化流程，并基于该蛋白建立针对癌症治疗的小分子抑制剂高通量筛选平台。
**4. "PNO1 as a Novel Biomarker: Recombinant Protein-Based Clinical Assay Development"**
*作者：Wang H, et al.*
摘要：开发基于重组人PNO1蛋白的ELISA检测方法，探讨其在肿瘤患者血清中的表达水平及其作为诊断标志物的潜力。
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**注意**：以上为模拟示例，实际文献需通过PubMed(https://pubmed.ncbi.nlm.nih.gov)、Google Scholar或Web of Science检索获取。建议使用关键词“recombinant PNO1”“human PNO1 expression”等查找近期研究。
Recombinant human PNO1 (Partner of NOB1) protein is a key player in ribosome biogenesis, a critical cellular process essential for protein synthesis. PNO1 interacts with NOB1. an endonuclease involved in rRNA processing, to facilitate the maturation of the 18S rRNA subunit within the 40S ribosomal subunit. This collaboration occurs in the nucleolus, where precursor rRNA (pre-rRNA) is cleaved and processed into functional rRNA components. PNO1’s role includes stabilizing NOB1 and ensuring precise cleavage of pre-rRNA, thereby maintaining ribosomal assembly fidelity and cellular translational capacity.
Produced via recombinant DNA technology in bacterial or eukaryotic expression systems, recombinant PNO1 retains structural and functional integrity for in vitro studies. Its applications span biochemical assays, structural analysis (e.g., X-ray crystallography), and functional studies to dissect ribosome assembly mechanisms. Dysregulation of PNO1 has been linked to developmental disorders and cancers, as aberrant ribosome biogenesis can drive uncontrolled cell proliferation. Recent research highlights PNO1’s potential as a biomarker or therapeutic target, particularly in malignancies with elevated ribosome activity. Studies also explore its interactions with other ribosome assembly factors, shedding light on conserved pathways across eukaryotes. Despite progress, detailed mechanisms of PNO1’s regulatory roles and post-translational modifications remain under investigation.
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