| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PNPLA4 |
| Uniprot No | P41247 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-253 aa |
| 活性数据 | MKHINLSFAACGFLGIYHLGAASALCRHGKKLVKDVKAFAGASAGSLVASVLLTAPEKIEECNQFTYKFAEEIRRQSFGAVTPGYDFMARLRSGMESILPPSAHELAQNRLHVSITNAKTRENHLVSTFSSREDLIKVLLASSFVPIYAGLKLVEYKGQKWVDGGLTNALPILPVGRTVTISPFSGRLDISPQDKGQLDLYVNIAKQDIMLSLANLVRLNQALFPPSKRKMESLYQCGFDDTVKFLLKENWFE |
| 分子量 | 29.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人PNPLA4蛋白的示例参考文献(注：文献为虚构示例，实际文献请通过数据库检索)：
1. **文献名称**: "Expression and functional characterization of recombinant human PNPLA4 in lipid metabolism"
**作者**: Zhang Y, et al.
**摘要**: 研究利用昆虫细胞系统成功表达重组人PNPLA4蛋白，并证实其具有磷脂酶活性，参与调节肝细胞内的脂质分解代谢。
2. **文献名称**: "Structural insights into PNPLA4 catalysis through recombinant protein crystallization"
**作者**: Müller R, et al.
**摘要**: 通过重组PNPLA4蛋白的晶体结构解析，揭示其催化域的关键氨基酸残基及与底物结合机制，为靶向药物设计提供依据。
3. **文献名称**: "Recombinant PNPLA4 production in E. coli and its role in adipose tissue inflammation"
**作者**: Kim S, et al.
**摘要**: 开发了大肠杆菌高效表达重组PNPLA4的方法，并证明该蛋白通过调控炎症因子分泌影响脂肪组织的慢性炎症过程。
4. **文献名称**: "Functional analysis of PNPLA4 variants using recombinant protein assays"
**作者**: Chen L, et al.
**摘要**: 构建重组PNPLA4突变体，通过体外酶活实验评估遗传突变对其功能的影响，发现某些变异与代谢综合征风险相关。
**建议**：可访问 **PubMed** 或 **Google Scholar**，输入关键词 `recombinant PNPLA4` 或 `PNPLA4 protein function` 获取真实文献。
Recombinant human PNPLA4 protein, derived from the patatin-like phospholipase domain-containing 4 (PNPLA4) gene, is a member of the PNPLA family of enzymes characterized by their conserved patatin-like α/β hydrolase domain. PNPLA4. also known as GS2 or iPLA2η, shares structural homology with adipose triglyceride lipase (ATGL/PNPLA2) and exhibits hydrolytic activity toward lipid substrates, though its specific biological role remains less defined compared to other family members. Expressed in various tissues, including liver, adipose, and skeletal muscle, PNPLA4 is implicated in lipid metabolism, membrane remodeling, and potentially cellular signaling. Studies suggest it may regulate glycerophospholipid homeostasis and interact with lipid droplets, though mechanistic details are still under investigation. Recombinant PNPLA4 protein is typically produced using heterologous expression systems (e.g., Escherichia coli or mammalian cells) for functional studies, structural analysis, and enzymatic assays. Its applications span basic research in metabolic disorders (e.g., non-alcoholic fatty liver disease) and pharmacological studies targeting lipid-associated pathways. Current research focuses on clarifying its substrate preferences, regulatory mechanisms, and potential links to human pathologies, as conflicting reports about its enzymatic activity and physiological relevance persist in the literature.
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