| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STH |
| Uniprot No | Q8IWL8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-128aa |
| 氨基酸序列 | MSEGGGQVSCIFAAPTRLCRWPALIECGVNLTQPLCEWMIQVARDRTLSLAWEVASLLTLSSSEVGLEGVGTIWPSSYSSEESSRNGAEQGRQLSIEGPFQGQNCPSHPAAALPLPMRGESQATSCQV |
| 预测分子量 | 29.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STH重组蛋白的3条示例参考文献(内容为虚构，仅供格式参考)：
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1. **文献名称**：*Expression and Purification of Recombinant STH Protein in Pichia pastoris*
**作者**：Chen L. et al.
**摘要**：本研究利用毕赤酵母表达系统高效生产重组STH蛋白，通过优化诱导条件和纯化工艺，获得高纯度且具有生物活性的STH蛋白，为后续功能研究奠定基础。
2. **文献名称**：*STH Recombinant Protein Enhances Wound Healing in Diabetic Mice*
**作者**：Wang X. et al.
**摘要**：探讨重组STH蛋白对糖尿病模型小鼠皮肤创面愈合的促进作用，发现其通过调控炎症因子和促进血管生成加速修复过程。
3. **文献名称**：*Structural Characterization and Functional Analysis of STH Recombinant Protein*
**作者**：Kim S. et al.
**摘要**：通过X射线晶体学解析STH重组蛋白的三维结构，结合体外实验证实其与特定受体结合并激活下游信号通路，揭示其分子作用机制。
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注：以上文献为示例性质，实际研究中请依据具体领域补充真实参考文献。
**Background of STH Recombinant Protein**
STH recombinant protein refers to a genetically engineered form of the *Streptococcal hemagglutinin* (STH) protein, derived from *Streptococcus pyogenes*, a pathogenic bacterium. STH is a surface-exposed adhesion molecule that facilitates bacterial colonization and immune evasion by binding to host extracellular matrix components, such as fibronectin and collagen. Its role in virulence and host-pathogen interactions has made it a target for therapeutic and diagnostic research.
The recombinant version is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and scalability. By cloning the *sth* gene into expression vectors, scientists can produce STH protein without pathogenic risks, enabling safe study of its structure and function. Recombinant STH retains the native protein’s binding properties, making it valuable for developing anti-adhesion therapies, vaccines, or diagnostic tools to combat streptococcal infections.
In research, STH recombinant protein aids in elucidating mechanisms of bacterial adherence, immune modulation, and potential cross-reactivity with host tissues, which is critical for understanding diseases like rheumatic fever or necrotizing fasciitis. Additionally, it serves as an antigen in antibody production or serological assays to detect streptococcal infections.
Challenges include optimizing expression conditions to ensure proper folding and post-translational modifications. Despite this, recombinant STH represents a safer, reproducible tool for advancing both basic microbiology and applied clinical solutions against *S. pyogenes*-related diseases. Ongoing studies aim to harness its properties for novel therapeutics or as a component in multivalent vaccines targeting streptococcal pathogens.
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