| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSCD3 |
| Uniprot No | O43739 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400 aa |
| 活性数据 | MDEDGGGEGG GVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL GERDEFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIA MNRGINEGGD LPEELLRNLY ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR VVEGNHVVYR ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK |
| 分子量 | 46.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的3-4条关于重组人PSCD3蛋白的参考文献示例(需根据实际研究调整)：
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1. **文献名称**: *Expression and Functional Characterization of Recombinant Human PSCD3 in Cancer Cell Lines*
**作者**: Chen, L. et al. (2022)
**摘要**: 本研究在HEK293细胞中成功表达了重组人PSCD3蛋白，发现其通过调节PI3K/AKT信号通路抑制肿瘤细胞生长，并通过蛋白质相互作用实验验证其与凋亡相关蛋白Bax的结合活性。
2. **文献名称**: *Crystal Structure and Enzymatic Activity of PSCD3: Insights into Its Phosphatase-like Domain*
**作者**: Nakamura, T. et al. (2019)
**摘要**: 通过昆虫细胞表达系统纯化PSCD3蛋白，解析其三维结构并鉴定其假性磷酸酶活性，揭示了该蛋白在金属离子结合及底物识别中的关键位点，为靶向药物设计提供结构基础。
3. **文献名称**: *PSCD3 Modulates Neuronal Differentiation via MAPK Signaling*
**作者**: Park, S. et al. (2020)
**摘要**: 利用重组PSCD3蛋白处理神经干细胞，发现其通过激活ERK1/2通路促进神经元分化，并证明其N端结构域对功能至关重要，提示其在神经发育疾病中的潜在作用。
4. **文献名称**: *Proteomic Analysis of PSCD3-Binding Partners in Immune Regulation*
**作者**: Wang, Q. et al. (2021)
**摘要**: 采用重组PSCD3蛋白进行免疫共沉淀-质谱分析，发现其与STAT3和NF-κB通路蛋白相互作用，实验表明PSCD3通过抑制炎症因子释放调控巨噬细胞极化，可能参与自身免疫疾病机制。
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**备注**：以上文献为模拟生成，实际研究中请通过PubMed、Google Scholar等平台以**"PSCD3"、"recombinant PSCD3"**或**"phosphatase domain containing 3"**为关键词检索，并核查基因别名(如是否指代*PIP4K2C*等)。若研究稀缺，建议扩大至同家族蛋白(如PSCD1/PSCD2)或相关功能领域文献。
**Background of Recombinant Human PSCD3 Protein**
Pleckstrin Homology and Sec7 Domain-containing protein 3 (PSCD3), also known as cytohesin-3. is a member of the cytohesin family of guanine nucleotide exchange factors (GEFs). It plays a critical role in regulating intracellular signaling and membrane trafficking by activating ADP-ribosylation factors (ARFs), small GTPases involved in vesicle formation and cytoskeletal organization. The protein features a conserved Sec7 domain responsible for GEF activity, a pleckstrin homology (PH) domain for membrane targeting, and a coiled-coil region mediating protein interactions.
PSCD3 is implicated in diverse cellular processes, including cell migration, adhesion, and immune response modulation. Dysregulation of PSCD3 has been linked to pathologies such as cancer metastasis, neurological disorders, and immune dysregulation. Recombinant human PSCD3 protein, produced via bacterial or mammalian expression systems, is widely used to study its biochemical properties, structure-function relationships, and interaction partners. Its application spans drug discovery, mechanistic studies of ARF-dependent pathways, and exploration of therapeutic targets for PSCD3-associated diseases. Research on recombinant PSCD3 enhances understanding of intracellular trafficking dynamics and its broader implications in health and disease.
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