| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRY2 |
| Uniprot No | O14603 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-147 aa |
| 活性数据 | MGATGLGFLL SWRQDNLNGT DCQGCNILYF SETTGSMCSE LSLNRGLEAR RKKDLKDSFL WRYGKVGCIS LPLREMTAWI NPPQISEIFQ GYHQRVHGAD ALSLQTNSLR SRLSSQCLGQ SFLLRTLERG RGFRALGDIC GHVHEED |
| 分子量 | 16.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRY2蛋白的3篇模拟参考文献示例(注：文献为虚构，仅用于示例格式)：
1. **文献名称**：*"Recombinant Human PRY2 Protein Promotes Tumor Growth by Regulating Apoptosis in Hepatocellular Carcinoma"*
**作者**：Zhang, L. et al. (2021)
**摘要**：本研究成功表达并纯化了重组人PRY2蛋白，发现其在肝癌细胞系中高表达可通过抑制Bax/Bcl-2通路减少细胞凋亡，促进肿瘤增殖。
2. **文献名称**：*"Structural and Functional Characterization of PRY2 in Sperm Development"*
**作者**：Smith, J. & Wang, Y. (2019)
**摘要**：通过重组PRY2蛋白体外实验，揭示其与SPATA16蛋白相互作用，调控精子顶体形成，基因敲除小鼠模型证实PRY2缺失导致不育。
3. **文献名称**：*"PRY2 Binds to Viral RNA and Modulates Innate Immune Response"*
**作者**：Chen, H. et al. (2020)
**摘要**：利用重组PRY2蛋白的RNA结合实验，发现其通过识别病毒RNA激活RIG-I信号通路，增强Ⅰ型干扰素分泌，提示其在抗病毒免疫中的潜在作用。
如需真实文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词 "recombinant human PRY2 protein" 或 "PRY2 function"。
**Background of Recombinant Human PRY2 Protein**
Recombinant human PRY2 (POBP/PRY domain-containing protein 2) is a protein of interest in reproductive biology and cellular regulation. The PRY2 gene, located on the Y chromosome, encodes a testis-specific protein predominantly expressed during spermatogenesis. It belongs to the POBP/PRY family, characterized by conserved PHD (plant homeodomain)-like zinc-binding motifs, which are often implicated in chromatin remodeling and transcriptional regulation.
PRY2 is primarily studied in the context of male infertility, as its dysfunction or abnormal expression has been linked to impaired sperm development, particularly in cases of azoospermia (absence of sperm in semen). The protein is thought to play a role in chromatin condensation during spermiogenesis, ensuring proper DNA packaging in sperm heads. Additionally, PRY2 may interact with other testis-specific proteins to regulate apoptosis and eliminate defective germ cells.
Recombinant PRY2 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*)**, enabling structural and functional studies. Its recombinant form allows researchers to explore molecular mechanisms underlying infertility, develop diagnostic biomarkers, or screen therapeutic agents targeting spermatogenic disorders. Further research aims to clarify its interactions with other Y chromosome-encoded proteins and its potential role as an autoantigen in immune-mediated infertility.
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