| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRSS8 |
| Uniprot No | Q16651 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343 aa |
| 活性数据 | MAQKGVLGPGQLGAVAILLYLGLLRSGTGAEGAEAPCGVAPQARITGGSSAVAGQWPWQVSITYEGVHVCGGSLVSEQWVLSAAHCFPSEHHKEAYEVKLGAHQLDSYSEDAKVSTLKDIIPHPSYLQEGSQGDIALLQLSRPITFSRYIRPICLPAANASFPNGLHCTVTGWGHVAPSVSLLTPKPLQQLEVPLISRETCNCLYNIDAKPEEPHFVQEDMVCAGYVEGGKDACQGDSGGPLSCPVEGLWYLTGIVSWGDACGARNRPGVYTLASSYASWIQSKVTELQPRVVPQTQESQPDSNLCGSHLAFSSAPAQGLLRPILFLPLGLALGLLSPWLSEH |
| 分子量 | 63.47 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRSS8蛋白的3篇参考文献，简要概括文献内容：
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1. **文献名称**: *Prostasin, a novel epithelial sodium channel–regulating protease*
**作者**: Vallet V., et al.
**摘要**: 该研究首次证明了PRSS8(prostasin)作为上皮钠离子通道(ENaC)的正向调节因子，通过重组蛋白表达揭示其在高盐环境下的调节作用，可能影响高血压发病机制。
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2. **文献名称**: *Recombinant human prostasin: production, activation, and characterization*
**作者**: Shipway A., et al.
**摘要**: 描述利用哺乳动物细胞表达系统生产重组人PRSS8蛋白的方法，分析其酶活性及对底物的特异性切割能力，为开发蛋白酶抑制剂提供基础。
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3. **文献名称**: *Role of prostasin in maintaining lung epithelial barrier function*
**作者**: Chen L.M., et al.
**摘要**: 通过重组PRSS8蛋白体外实验，验证其通过调控细胞连接蛋白增强肺上皮屏障完整性，为呼吸系统疾病(如ARDS)的治疗提供新靶点。
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(注：上述文献信息为虚拟示例，实际检索需查询PubMed或Google Scholar数据库。)
**Background of Recombinant Human PRSS8 Protein**
PRSS8. also known as prostasin, is a trypsin-like serine protease encoded by the *PRSS8* gene. Belonging to the peptidase S1 family, it is expressed in various epithelial tissues, including the skin, kidney, lungs, and prostate. PRSS8 exists in both membrane-bound and secreted forms, playing critical roles in maintaining epithelial barrier integrity, electrolyte balance, and cellular differentiation. It regulates sodium transport by activating the epithelial sodium channel (ENaC) and participates in extracellular matrix remodeling through interactions with proteolytic cascades.
Dysregulation of PRSS8 is linked to pathological conditions such as hypertension, cystic fibrosis, and cancer. In cancer, PRSS8 exhibits dual roles, acting as either a tumor suppressor or promoter depending on context. For example, its downregulation correlates with poor prognosis in prostate and lung cancers, while overexpression is observed in certain ovarian and colorectal cancers. Additionally, PRSS8 has emerged as a potential biomarker for preeclampsia due to its altered expression in placental tissues.
Recombinant human PRSS8 protein, produced via genetic engineering in systems like mammalian or insect cells, retains enzymatic activity and structural fidelity. It serves as a vital tool for studying protease mechanisms, protein-protein interactions, and therapeutic targeting. Its applications span basic research, drug discovery, and biomarker development, reflecting its significance in both physiological and pathological processes. Ongoing research aims to clarify its context-dependent functions and therapeutic potential.
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