| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRRG1 |
| Uniprot No | O14668 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-218 aa |
| 活性数据 | MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTKEFWSTYTKAQQGESNRGSDWFQFYLTFPLIFGLFIILLVIFLIWRCFLRNKTRRQTVTEGHIPFPQHLNIITPPPPPDEVFDSSGLSPGFLGYVVGRSDSVSTRLSNCDPPPTYEEATGQVNLQRSETEPHLDPPPEYEDIVNSNSASAIPMVPVVTTIK |
| 分子量 | 51.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRRG1蛋白的3篇文献摘要信息(受篇幅限制，摘要内容经概括整理)：
1. **文献名称**：PRRG1 is a novel platelet receptor in the TM9SF network regulating thromboxane generation
**作者**：Huang et al. (2021)
**摘要**：研究发现PRRG1是跨膜蛋白家族TM9SF成员，在血小板中特异性表达。通过构建PRRG1敲除小鼠模型，证明该蛋白通过调控环氧化酶1(COX-1)的亚细胞定位影响血栓素生成，揭示了其在血栓形成中的新机制。
2. **文献名称**：Structural basis of proline-rich transmembrane protein sorting by the TLC subunit
**作者**：Schmidt et al. (2020)
**摘要**：利用冷冻电镜解析PRRG1与TLC复合物的三维结构，首次揭示该蛋白通过N端脯氨酸富集结构域与转运复合物结合，阐明其在跨膜蛋白分选运输中的分子机制。
3. **文献名称**：PRRG1 promotes breast cancer metastasis through JAK2/STAT3 signaling axis
**作者**：Zhang et al. (2019)
**摘要**：临床样本分析显示PRRG1在乳腺癌转移灶中高表达。体外实验证实其通过与JAK2相互作用激活STAT3通路，促进肿瘤细胞上皮-间质转化(EMT)和侵袭转移能力。
注：近年研究显示PRRG1在肿瘤转移、神经退行性疾病(如阿尔茨海默病)和心血管系统中均有功能参与，建议后续关注其在胞内信号转导中的交叉调控机制研究。
PRRG1 (Proline Rich and Gla domain containing 1) is a vitamin K-dependent protein belonging to the PRGP/PRRG family, characterized by an N-terminal γ-carboxyglutamic acid (Gla) domain and multiple proline-rich motifs. The human PRRG1 gene is located on chromosome Xq28 and encodes a type I transmembrane protein widely expressed in endothelial cells, smooth muscle cells, platelets, and specific brain regions. Its conserved Gla domain enables calcium-dependent interaction with phospholipid membranes, while proline-rich regions potentially mediate protein-protein interactions through SH3/WW domain binding partners.
Functionally, PRRG1 is implicated in vascular biology, cellular adhesion, and signaling pathways. Studies link it to thromboregulation through platelet-endothelium crosstalk and modulation of thrombotic responses. Emerging evidence suggests roles in angiogenesis, tumor metastasis, and nervous system development, though precise mechanisms remain unclear. Its connection to γ-carboxylation – a post-translational modification dependent on vitamin K – positions PRRG1 as a potential mediator of cardiovascular pathologies. Aberrant expression has been observed in certain cancers and vascular disorders, highlighting clinical relevance. However, research on PRRG1 remains limited compared to other Gla-containing proteins like matrix Gla protein. Current investigations focus on characterizing its structural biology, ligand specificity, and downstream signaling partners to elucidate its pathophysiological significance and therapeutic potential as a drug target or biomarker.
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