| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRSS25 |
| Uniprot No | O43464 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 134-458 aa |
| 活性数据 | MAVPSPPPASPPSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNAGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTEGSHHHHHH |
| 分子量 | 36 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRSS25蛋白的3-4条参考文献的简要列举：
1. **标题**: *Recombinant human PRSS25 protein promotes endothelial cell angiogenesis through protease-activated receptor signaling*
**作者**: Wang L, et al.
**摘要**: 本研究成功在昆虫细胞系统中表达了重组人PRSS25蛋白，并证明其通过激活PAR2受体增强内皮细胞血管生成能力，提示其在组织修复中的潜在应用。
2. **标题**: *PRSS25 regulates extracellular matrix remodeling via cleavage of fibronectin in hepatic fibrosis*
**作者**: Kim S, et al.
**摘要**: 利用哺乳动物细胞表达的重组PRSS25.研究发现其能特异性切割纤连蛋白，促进肝星状细胞活化，为肝纤维化机制提供了新见解。
3. **标题**: *Expression and purification of recombinant PRSS25 in Pichia pastoris: Biochemical characterization and substrate specificity profiling*
**作者**: Gonzalez R, et al.
**摘要**: 报道了在毕赤酵母中高效分泌表达PRSS25的工艺优化，通过质谱分析鉴定了其对富含脯氨酸肽段的底物偏好性，为其功能研究奠定基础。
4. **标题**: *PRSS25 as a novel regulator of innate immunity: Insights from recombinant protein-based functional assays*
**作者**: Müller J, et al.
**摘要**: 重组PRSS25被证实可降解细菌鞭毛蛋白，抑制TLR5信号通路，提示其在先天免疫防御中的新角色。
**说明**：以上内容为模拟生成，实际文献需通过学术数据库检索核实。若需具体文献，建议在PubMed或Web of Science中以“PRSS25 recombinant”或“PRSS25 protease”为关键词检索。
**Background of Recombinant Human PRSS25 Protein**
Recombinant human PRSS25. also known as serine protease 25. is a protein encoded by the *PRSS25* gene, belonging to the trypsin-like serine protease family. These proteases are characterized by a conserved catalytic triad (histidine, aspartate, and serine) and play roles in diverse physiological processes, including tissue remodeling, immune response, and cellular signaling. PRSS25 is classified as a type II transmembrane serine protease (TTSP) with a structure comprising an N-terminal transmembrane domain, a stem region, and a C-terminal serine protease catalytic domain. While its precise physiological functions remain under investigation, PRSS25 is implicated in extracellular matrix degradation, cell adhesion, and inflammatory regulation, potentially influencing pathways related to cancer metastasis, wound healing, and immune modulation.
Recombinant PRSS25 is typically produced in mammalian or insect expression systems to ensure proper post-translational modifications and enzymatic activity. Purification methods like affinity chromatography yield high-purity protein for research applications. Studies focus on elucidating its substrate specificity, regulatory mechanisms, and role in diseases such as cancers, fibrosis, or infectious diseases. For instance, PRSS25 may interact with viral entry pathways or modulate cytokine signaling, highlighting its therapeutic or diagnostic potential. However, its expression patterns and mechanistic contributions require further validation, necessitating functional assays and *in vivo* models. Current research aims to unravel PRSS25’s biological significance and translational relevance in human health.
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