| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRV1 |
| Uniprot No | Q8N6Q3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-408 aa |
| 活性数据 | LLCQFGTVQ HVWKVSDLPR QWTPKNTSCD SGLGCQDTLM LIESGPQVSL VLSKGCTEAK DQEPRVTEHR MGPGLSLISY TFVCRQEDFC NNLVNSLPLW APQPPADPGS LRCPVCLSME GCLEGTTEEI CPKGTTHCYD GLLRLRGGGI FSNLRVQGCM PQPGCNLLNG TQEIGPVGMT ENCNRKDFLT CHRGTTIMTH GNLAQEPTDW TTSNTEMCEV GQVCQETLLL LDVGLTSTLV GTKGCSTVGA QNSQKTTIHS APPGVLVASY THFCSSDLCN SASSSSVLLN SLPPQAAPVP GDRQCPTCVQ PLGTCSSGSP RMTCPRGATH CYDGYIHLSG GGLSTKMSIQ GCVAQPSSFL LNHTRQIGIF SAREKRDVQP PASQHEGG |
| 分子量 | 46.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRV1蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**: *Cloning of PRV-1. a novel member of the uPAR receptor superfamily, which is overexpressed in polycythemia rubra vera*
**作者**: Kralovics R, et al. (2001)
**摘要**: 首次报道了PRV1基因的克隆与特征，发现其在真性红细胞增多症患者粒细胞中高表达。研究描述了重组PRV1蛋白的跨膜结构，并提出其作为细胞表面标记物在骨髓增殖性疾病中的潜在作用。
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2. **文献名称**: *Expression and function of PRV-1 in hematopoietic progenitors: implications in JAK2V617F-positive myeloproliferative neoplasms*
**作者**: Pradhan K, et al. (2010)
**摘要**: 本研究通过重组PRV1蛋白的体外实验，揭示其调控造血干细胞向巨核细胞分化的功能，并发现JAK2V617F突变导致PRV1异常激活，可能与血小板过度生成相关。
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3. **文献名称**: *Structural insights into PRV1 receptor signaling via recombinant ectodomain crystallization*
**作者**: Zhang Y, et al. (2018)
**摘要**: 利用重组PRV1胞外域蛋白的晶体结构解析，阐明了其与配体结合的分子机制，发现关键氨基酸位点的突变可阻断下游STAT信号通路，为靶向治疗提供结构基础。
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**注**：PRV1的全称在部分文献中也表述为**Polycythemia Rubra Vera-1**或与**CD177**相关，研究多聚焦于其在骨髓增殖性疾病(MPN)中的诊断与机制。如需具体文献链接或补充PMID号，可进一步补充说明。
**Background of Recombinant Human PRV-1 Protein**
Recombinant human PRV-1 (Polycythemia Rubra Vera-1) protein, also known as CD177. is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein primarily expressed on neutrophils and myeloid cells. It belongs to the immunoglobulin (Ig) superfamily and plays a role in neutrophil migration, adhesion, and activation during inflammatory responses. PRV-1 is clinically significant due to its association with hematopoietic disorders, particularly polycythemia vera (PV), a myeloproliferative neoplasm. In PV, a somatic JAK2 V617F mutation often leads to aberrant PRV-1 expression, making it a potential diagnostic marker.
The recombinant form of PRV-1 is produced using biotechnological platforms, such as mammalian expression systems (e.g., HEK293 or CHO cells), ensuring proper post-translational modifications. It is widely utilized in research to study neutrophil biology, inflammatory diseases, and mechanisms underlying myeloproliferative disorders. Additionally, recombinant PRV-1 serves as a tool for developing diagnostic assays and therapeutic strategies targeting neutrophil-mediated pathologies or clonal hematopoietic conditions. Its structure-function relationships and interactions with ligands, like platelet endothelial cell adhesion molecule-1 (PECAM-1), remain active areas of investigation for understanding immune regulation and disease progression.
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