| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSD2 |
| Uniprot No | Q9BQI7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-771 aa |
| 活性数据 | MEEDKLLSAVPEEGDATRDPGPEPEEEPGVRNGMASEGLNSSLCSPGHERRGTPADTEEPTKDPDVAFHGLSLGLSLTNGLALGPDLNILEDSAESRPWRAGVLAEGDNASRSLYPDAEDPQLGLDGPGEPDVRDGFSATFEKILESELLRGTQYSSLDSLDGLSLTDESDSCVSFEAPLTPLIQQRARDSPEPGAGLGIGDMAFEGDMGAAGGDGELGSPLRRSISSSRSENVLSRLSLMAMPNGFHEDGPQGPGGDEDDDEEDTDKLLNSASDPSLKDGLSDSDSELSSSEGLEPGSADPLANGCQGVSEAAHRLARRLYHLEGFQRCDVARQLGKNNEFSRLVAGEYLSFFDFSGLTLDGALRTFLKAFPLMGETQERERVLTHFSRRYCQCNPDDSTSEDGIHTLTCALMLLNTDLHGHNIGKKMSCQQFIANLDQLNDGQDFAKDLLKTLYNSIKNEKLEWAIDEDELRKSLSELVDDKFGTGTKKVTRILDGGNPFLDVPQALSATTYKHGVLTRKTHADMDGKRTPRGRRGWKKFYAVLKGTILYLQKDEYRPDKALSEGDLKNAIRVHHALATRASDYSKKSNVLKLKTADWRVFLFQAPSKEEMLSWILRINLVAAIFSAPAFPAAVSSMKKFCRPLLPSCTTRLCQEEQLRSHENKLRQLTAELAEHRCHPVERGIKSKEAEEYRLKEHYLTFEKSRYETYIHLLAMKIKVGSDDLERIEARLATLEGDDPSLRKTHSSPALSQGHVTGSKTTKDATGPDT |
| 分子量 | 111.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PSD2蛋白的虚构参考文献示例(注：PSD2研究较少，实际文献可能需替换为相关领域)：
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1. **文献名称**：Expression and Purification of Recombinant Human PSD2 in E. coli for Structural Analysis
**作者**：Li X. et al. (2018)
**摘要**：该研究成功在大肠杆菌中克隆并表达了重组人PSD2蛋白，优化表达条件并通过亲和层析纯化，获得高纯度蛋白用于后续的X射线晶体学分析，为理解其结构功能奠定了基础。
2. **文献名称**：Functional Characterization of PSD2 in Synaptic Plasticity Using Mammalian Cell Systems
**作者**：Zhang Y. et al. (2020)
**摘要**：通过哺乳动物细胞(HEK293)表达重组人PSD2.发现其与NMDA受体亚基直接互作，并在突触可塑性调控中发挥关键作用，提示其可能作为神经退行性疾病的潜在治疗靶点。
3. **文献名称**：Crystal Structure of Human PSD2 Reveals a Novel Protein-Binding Domain
**作者**：Wang T. et al. (2019)
**摘要**：解析了重组人PSD2的晶体结构(分辨率2.8 Å)，发现其N端含有一个未报道的蛋白质结合域，可能参与突触后信号复合物的组装，为研究神经发育机制提供结构依据。
4. **文献名称**：Development of a High-Throughput Screening Assay for PSD2 Modulators
**作者**：Liu R. et al. (2021)
**摘要**：利用重组人PSD2蛋白建立基于荧光偏振的药物筛选平台，筛选出多个小分子候选化合物，为治疗PSD2相关认知障碍疾病提供了新方向。
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**注意事项**：
- PSD2的生物学功能研究较少，若实际检索困难，建议确认名称准确性(如是否涉及脂质代谢中的PHD结构域蛋白2.或是否为PSD-95等类似蛋白的笔误)。
- 实际应用中需通过数据库(如PubMed、Google Scholar)检索真实文献。
Recombinant human PSD-95 (Postsynaptic density protein 95), also known as PSD2. is a critical scaffolding protein enriched in the postsynaptic density (PSD) of excitatory neurons. Belonging to the membrane-associated guanylate kinase (MAGUK) family, it plays a pivotal role in organizing synaptic signaling complexes by interacting with NMDA receptors, AMPA receptors, and downstream signaling molecules. PSD-95 is essential for synaptic plasticity, a cellular basis for learning and memory, and its dysregulation has been linked to neurological disorders such as Alzheimer’s disease, schizophrenia, and autism spectrum disorders.
Recombinant PSD2 is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) to study its structure-function relationships, protein-protein interactions, and therapeutic potential. Its modular domains (PDZ, SH3. GK) enable multidomain engineering for research applications, including drug discovery targeting synaptic dysfunction. Advanced purification techniques ensure high-purity, bioactive recombinant PSD2 for biochemical assays, structural studies, and in vitro modeling of synaptic pathways. Current research focuses on modulating PSD-95 interactions to develop neuroprotective strategies or cognitive enhancers, highlighting its significance in both basic neuroscience and translational medicine.
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