| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PUSL1 |
| Uniprot No | Q8N0Z8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-303 aa |
| 活性数据 | MSSAPASGSV RARYLVYFQY VGTDFNGVAA VRGTQRAVGV QNYLEEAAER LNSVEPVRFT ISSRTDAGVH ALSNAAHLDV QRRSGRPPFP PEVLAEALNT HLRHPAIRVL RAFRVPSDFH ARHAATSRTY LYRLATGCHR RDELPVFERN LCWTLPADCL DMVAMQEAAQ HLLGTHDFSA FQSAGSPVPS PVRTLRRVSV SPGQASPLVT PEESRKLRFW NLEFESQSFL YRQVRRMTAV LVAVGLGALA PAQVKTILES QDPLGKHQTR VAPAHGLFLK SVLYGNLGAA SCTLQGPQFG SHG |
| 分子量 | 33.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假想研究整理的重组人PUSL1蛋白相关参考文献示例(注意：PUSL1的实际研究较少，以下内容为模拟生成)：
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1. **文献名称**: *Functional Characterization of Recombinant Human PUSL1 in tRNA Modification*
**作者**: Zhang et al.
**摘要**: 报道了人源PUSL1蛋白在大肠杆菌中的重组表达与纯化，证实其具有假尿苷合成酶活性，参与线粒体tRNA修饰，并解析了酶活关键结构域。
2. **文献名称**: *PUSL1 Knockout Alters Mitochondrial Function in Mammalian Cells*
**作者**: Lee et al.
**摘要**: 通过重组PUSL1蛋白回补实验，证明其在线粒体代谢中的必要性，缺失导致细胞氧化磷酸化异常，提示与遗传性线粒体疾病的潜在关联。
3. **文献名称**: *Crystal Structure Analysis of Human PUSL1 Protein*
**作者**: Watanabe et al.
**摘要**: 首次解析重组PUSL1的晶体结构，揭示其催化口袋的保守性，为设计针对PUSL1相关疾病的抑制剂提供了结构基础。
4. **文献名称**: *Development of PUSL1-Specific Monoclonal Antibodies Using Recombinant Protein*
**作者**: Chen et al.
**摘要**: 利用重组PUSL1蛋白制备高特异性抗体，验证了其在肝细胞癌中的异常表达，提出其作为生物标志物的潜在价值。
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**注意**：PUSL1(Pseudouridine Synthase Like 1)的实际研究较少，上述文献为模拟示例。建议通过PubMed或Google Scholar以"PUSL1 protein" "recombinant PUSL1"为关键词检索最新进展。
**Background of Recombinant Human PUSL1 Protein**
Recombinant human pseudouridine synthase-like 1 (PUSL1) protein is a genetically engineered enzyme belonging to the pseudouridine synthase family, which catalyzes the isomerization of uridine to pseudouridine (Ψ) in RNA molecules. Pseudouridylation is a common post-transcriptional modification critical for RNA stability, structure, and function. PUSL1. also termed mitochondrial pseudouridine synthase, localizes primarily to mitochondria and is implicated in modifying mitochondrial tRNA and rRNA, thereby influencing mitochondrial translation and energy production.
Structurally, PUSL1 contains conserved catalytic domains characteristic of pseudouridine synthases, including the TruB and catalytic motifs essential for RNA binding and isomerase activity. Dysregulation of PUSL1 has been linked to mitochondrial dysfunction and human diseases, such as mitochondrial myopathy with lactic acidosis and sideroblastic anemia (MLASA), caused by mutations in the *PUSL1* gene. Recombinant PUSL1 is typically produced in *E. coli* or mammalian expression systems, enabling studies on its enzymatic mechanisms, RNA interaction networks, and therapeutic potential. Its recombinant form facilitates research into mitochondrial disorders, RNA biology, and the development of targeted therapies for metabolic diseases.
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