| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB43 |
| Uniprot No | Q86YS6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-212 aa |
| 活性数据 | MAGPGPGPGD PDEQYDFLFK LVLVGDASVG KTCVVQRFKT GAFSERQGST IGVDFTMKTL EIQGKRVKLQ IWDTAGQERF RTITQSYYRS ANGAILAYDI TKRSSFLSVP HWIEDVRKYA GSNIVQLLIG NKSDLSELRE VSLAEAQSLA EHYDILCAIE TSAKDSSNVE EAFLRVATEL IMRHGGPLFS EKSPDHIQLN SKDIGEGWGC GC |
| 分子量 | 23.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人RAB43蛋白相关的参考文献列表(虚构示例，仅供参考)：
1. **"RAB43 regulates Golgi-to-ER retrograde transport via interaction with COPI machinery"**
- **作者**: Smith J. et al.
- **摘要**: 本研究揭示了RAB43通过结合COPI复合体调控高尔基体到内质网的逆向运输过程，并证明其GTP酶活性对维持细胞器膜结构至关重要。
2. **"Recombinant human RAB43 expression and purification for structural analysis"**
- **作者**: Lee S. & Zhang Y.
- **摘要**: 报道了一种高效表达和纯化重组人RAB43蛋白的方法，结合X射线晶体学解析了其三维结构，为探究其功能机制提供了基础。
3. **"RAB43 modulates lysosomal exocytosis in macrophages during immune response"**
- **作者**: Garcia M. et al.
- **摘要**: 发现RAB43通过调控溶酶体胞吐作用影响巨噬细胞的抗原呈递能力，其缺失导致免疫反应异常，提示其在炎症和感染中的作用。
4. **"The role of RAB43 in cancer cell migration and invasion"**
- **作者**: Wang X. et al.
- **摘要**: 证实RAB43通过激活Wnt/β-catenin通路促进肿瘤细胞迁移和侵袭，靶向RAB43可抑制多种癌症模型的转移潜力。
---
注：上述内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索确认。
**Recombinant Human RAB43 Protein: Background**
RAB43. a member of the RAS-associated binding (RAB) family of small GTPases, plays a critical role in intracellular membrane trafficking and vesicle transport. These proteins act as molecular switches, cycling between active GTP-bound and inactive GDP-bound states to regulate organelle dynamics, cargo sorting, and secretory pathways. Specifically, RAB43 is implicated in Golgi apparatus organization, endosome-to-Golgi retrograde transport, and maintaining lysosomal function. Studies suggest its involvement in autophagy, cell signaling, and potentially immune responses, though its precise mechanisms remain under investigation.
The recombinant human RAB43 protein is artificially expressed and purified using systems like *E. coli* or mammalian cells, enabling researchers to study its structure, interactions, and biochemical activity *in vitro*. Its recombinant form often includes tags (e.g., GST, His-tag) for easier purification and detection. Dysregulation of RAB GTPases, including RAB43. has been linked to cancers, neurodegenerative disorders, and metabolic diseases, making this protein a target for therapeutic exploration. Recent research highlights RAB43's potential role in cancer cell proliferation and drug resistance, emphasizing its relevance in oncology.
Overall, recombinant RAB43 serves as a vital tool for deciphering its cellular functions and developing strategies to modulate RAB-related pathologies, bridging basic research and translational applications.
×
