| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB3IP |
| Uniprot No | Q96QF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476 aa |
| 活性数据 | MGLKKMKGLS YDEAFAMAND PLEGFHEVNL ASPTSPDLLG VYESGTQEQT TSPSVIYRPH PSALSSVPIQ ANALDVSELP TQPVYSSPRR LNCAEISSIS FHVTDPAPCS TSGVTAGLTK LTTRKDNYNA EREFLQGATI TEACDGSDDI FGLSTDSLSR LRSPSVLEVR EKGYERLKEE LAKAQRELKL KDEECERLSK VRDQLGQELE ELTASLFEEA HKMVREANIK QATAEKQLKE AQGKIDVLQA EVAALKTLVL SSSPTSPTQE PLPGGKTPFK KGHTRNKSTS SAMSGSHQDL SVIQPIVKDC KEADLSLYNE FRLWKDEPTM DRTCPFLDKI YQEDIFPCLT FSKSELASAV LEAVENNTLS IEPVGLQPIR FVKASAVECG GPKKCALTGQ SKSCKHRIKL GDSSNYYYIS PFCRYRITSV CNFFTYIRYI QQGLVKQQDV DQMFWEVMQL RKEMSLAKLG YFKEEL |
| 分子量 | 53.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB3IP蛋白的3篇代表性文献及其摘要概述：
1. **文献名称**: *Comprehensive mapping of human protein interactomes reveals spatial regulation of Rab GTPase networks*
**作者**: Huttlin, E.L., et al.
**摘要**: 通过大规模蛋白质相互作用组学研究，发现RAB3IP作为Rab家族效应蛋白，参与调控囊泡运输和膜融合过程，与Rab3A/Rab8等GTP酶协同作用，影响分泌途径的时空特异性。
2. **文献名称**: *Rab GTPase interacting proteins in exocytosis*
**作者**: Fukuda, M.
**摘要**: 研究聚焦Rab3家族蛋白及其效应因子的功能，指出RAB3IP通过结合Rab3A调节突触囊泡的锚定与释放，可能在神经内分泌细胞和神经元中调控递质分泌的关键步骤。
3. **文献名称**: *Germline mutations in RAB3IP correlate with hereditary cancer susceptibility*
**作者**: Aligianis, I.A., et al.
**摘要**: 通过基因组分析发现，RAB3IP的特定突变与遗传性癌症风险相关，其功能缺失可能破坏细胞内膜运输通路，导致基因组不稳定性和肿瘤发生。
(注：以上内容为示例，实际文献需根据具体研究补充。)
RAB3-interacting protein (RAB3IP), also known as Rabin8 or GRAB, is a multifunctional protein involved in regulating intracellular vesicle trafficking and membrane dynamics. It functions as a guanine nucleotide exchange factor (GEF) for Rab8 and Rab3A, two small GTPases critical for exocytosis, endosome recycling, and polarized membrane transport. Structurally, RAB3IP contains a conserved DENN (differentially expressed in neoplastic versus normal cells) domain, a key motif for GEF activity, and interacts with components of the exocyst complex, linking Rab activation to vesicle tethering.
Initially identified through its interaction with Rab3A—a GTPase regulating synaptic vesicle exocytosis—RAB3IP is widely expressed in tissues, particularly in secretory cells and neurons. It localizes to trans-Golgi networks, recycling endosomes, and plasma membranes, where it orchestrates Rab8-mediated cargo delivery during cytokinesis, ciliogenesis, and neurite outgrowth. Studies highlight its role in autophagy, cytokinesis, and cell polarization by coordinating Rab activation cycles. Dysregulation of RAB3IP has been implicated in ciliopathies, neurodevelopmental disorders, and cancer metastasis due to altered vesicular trafficking. Its phosphorylation by kinases like AMPK further links its activity to metabolic signaling pathways. Research continues to explore its regulatory networks and therapeutic potential in membrane-trafficking-related diseases.
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