| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RCOR1 |
| Uniprot No | Q9UKL0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4-485 aa |
| 活性数据 | MVEKGPEVSGKRRGRNNAAASASAAAASAAASAACASPAATAASGAAASSASAAAASAAAAPNNGQNKSLAAAAPNGNSSSNSWEEGSSGSSSDEEHGGGGMRVGPQYQAVVPDFDPAKLARRSQERDNLGMLVWSPNQNLSEAKLDEYIAIAKEKHGYNMEQALGMLFWHKHNIEKSLADLPNFTPFPDEWTVEDKVLFEQAFSFHGKTFHRIQQMLPDKSIASLVKFYYSWKKTRTKTSVMDRHARKQKREREESEDELEEANGNNPIDIEVDQNKESKKEVPPTETVPQVKKEKHSTQAKNRAKRKPPKGMFLSQEDVEAVSANATAATTVLRQLDMELVSVKRQIQNIKQTNSALKEKLDGGIEPYRLPEVIQKCNARWTTEEQLLAVQAIRKYGRDFQAISDVIGNKSVVQVKNFFVNYRRRFNIDEVLQEWEAEHGKEETNGPSNQKPVKSPDNSIKMPEEEDEAPVLDVRYASAS |
| 分子量 | 100.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为与重组人RCOR1蛋白相关的参考文献示例(注：文献标题与摘要内容为模拟，请根据实际检索验证)：
1. **标题**: *"RCOR1/LSD1 Complex Coordinates Epigenetic Landscapes for Neural Stem Cell Fate"*
**作者**: Andreu-Zornoza, A. et al. (2018)
**摘要**: 通过重组RCOR1与LSD1蛋白复合体的体外功能研究，揭示其在神经干细胞分化中调控染色质动态和关键基因沉默的作用。
2. **标题**: *"CoREST-dependent enzyme assembly modulates histone demethylation and gene repression"*
**作者**: Ballas, N. et al. (2005)
**摘要**: 采用重组RCOR1(CoREST)蛋白与组蛋白去乙酰化酶(HDAC)及甲基转移酶复合体，验证其协同调控靶基因抑制的分子机制。
3. **标题**: *"RCOR1-mediated transcriptional regulation promotes lung cancer progression"*
**作者**: You, J. et al. (2017)
**摘要**: 利用重组RCOR1蛋白进行体外功能实验，证实其通过调控Wnt信号通路关键因子促进肺癌细胞增殖与转移。
4. **标题**: *"Structural basis of RCOR1 interaction with REST in epigenetic silencing"*
**作者**: Lakowski, T.M. et al. (2001)
**摘要**: 通过重组蛋白互作实验解析RCOR1与REST转录因子的结构域结合模式，揭示其在神经元基因抑制中的分子基础。
**提示**：建议使用PubMed或Google Scholar以“RCOR1 recombinant protein”或“CoREST recombinant”为关键词检索最新文献，并优先选择涉及RCOR1功能机制、结构解析或疾病关联的研究。
RCOR1 (REST Corepressor 1), also known as CoREST, is a crucial epigenetic regulatory protein that interacts with the REST (RE1-Silencing Transcription Factor) complex to mediate gene silencing. Functioning as a scaffold protein, RCOR1 facilitates the assembly of chromatin-modifying complexes, including histone deacetylases (HDAC1/2) and lysine-specific demethylase 1 (LSD1/KDM1A), collectively termed the CoREST complex. This complex targets genes involved in neuronal development, differentiation, and cellular identity by modifying histone marks to establish repressive chromatin states.
RCOR1 plays a pivotal role in neurogenesis by suppressing non-neuronal genes during stem cell differentiation into neurons. It also regulates pluripotency in embryonic stem cells and maintains genomic stability. Beyond neurobiology, RCOR1 is implicated in cancer, where its dysregulation can either suppress or promote tumorigenesis depending on cellular context. For instance, it may act as a tumor suppressor by repressing oncogenic pathways or as an oncogene by silencing differentiation-promoting genes.
Genome-wide studies show RCOR1 binds to thousands of genomic sites, often co-localizing with REST at neuron-specific genes, but it also exhibits REST-independent functions. Structural studies reveal its conserved SANT domains enable interactions with DNA, histones, and partner proteins. Research on RCOR1 continues to uncover its multifaceted roles in epigenetics, development, and disease, making it a potential therapeutic target for neurological disorders and cancers.
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