| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REC14 |
| Uniprot No | Q9GZS3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-305 aa |
| 活性数据 | TNQYGILFK QEQAHDDAIW SVAWGTNKKE NSETVVTGSL DDLVKVWKWR DERLDLQWSL EGHQLGVVSV DISHTLPIAA SSSLDAHIRL WDLENGKQIK SIDAGPVDAW TLAFSPDSQY LATGTHVGKV NIFGVESGKK EYSLDTRGKF ILSIAYSPDG KYLASGAIDG IINIFDIATG KLLHTLEGHA MPIRSLTFSP DSQLLVTASD DGYIKIYDVQ HANLAGTLSG HASWVLNVAF CPDDTHFVSS SSDKSVKVWD VGTRTCVHTF FDHQDQVWGV KYNGNGSKIV SVGDDQEIHI YDCPI |
| 分子量 | 33.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人REC14蛋白的简要参考文献列表(注：REC14蛋白相关研究较少，以下内容为模拟示例，实际文献需根据具体研究补充)：
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1. **标题**: *Expression and Purification of Recombinant Human REC14 Protein in E. coli*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达了重组人REC14蛋白，并优化了纯化条件。该蛋白具有DNA结合活性，为后续功能研究提供了基础。
2. **标题**: *Structural Insights into REC14's Role in DNA Repair*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析了REC14蛋白的晶体结构，揭示了其与DNA损伤修复相关结构域的作用机制，证实其在同源重组中的关键功能。
3. **标题**: *Functional Analysis of REC14 in Mammalian Cell Cycle Regulation*
**作者**: Gupta R, et al.
**摘要**: 利用基因敲除技术，发现REC14蛋白通过调控ATM/ATR信号通路影响细胞周期检查点，缺失导致基因组不稳定性增加。
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**说明**：
- 若需真实文献，请通过PubMed/Google Scholar检索关键词“recombinant REC14 human”或相关功能描述，或确认蛋白名称准确性(如是否为RECQL家族或其他命名变体)。
- 部分研究可能以“REC14”作为别名(如与减数分裂重组相关蛋白)，需结合具体背景筛选。
Recombinant human REC14 (RH14) protein is a genetically engineered variant derived from the human Rec14 homolog, which plays a role in DNA repair and homologous recombination processes. Originally identified in *Schizosaccharomyces pombe*, Rec14 is implicated in meiotic recombination and chromosomal stability. The human homolog, though less characterized, is suggested to interact with recombination-associated proteins, potentially contributing to genome maintenance and error-free DNA repair.
Recombinant REC14 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enabling high-purity yields for functional studies. Its production facilitates investigations into mechanisms of DNA damage response, mitotic recombination, and cell cycle regulation. Studies suggest REC14 may act as a scaffold or modulator in multi-protein complexes, though exact molecular interactions remain under exploration.
Research applications include analyzing its role in cancer biology, particularly in contexts of genomic instability and chemotherapy resistance. Additionally, REC14’s involvement in meiosis makes it relevant for understanding infertility and developmental disorders. Current limitations include incomplete structural data and signaling pathway mapping. Ongoing efforts aim to delineate its post-translational modifications, tissue-specific expression, and therapeutic potential as a biomarker or drug target. This recombinant tool bridges gaps in understanding conserved DNA repair mechanisms across eukaryotes.
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