| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RDH5 |
| Uniprot No | Q92781 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-318 aa |
| 活性数据 | LPASNAF VFITGCDSGF GRLLALQLDQ RGFRVLASCL TPSGAEDLQR VASSRLHTTL LDITDPQSVQ QAAKWVEMHV KEAGLFGLVN NAGVAGIIGP TPWLTRDDFQ RVLNVNTMGP IGVTLALLPL LQQARGRVIN ITSVLGRLAA NGGGYCVSKF GLEAFSDSLR RDVAHFGIRV SIVEPGFFRT PVTNLESLEK TLQACWARLP PATQAHYGGA FLTKYLKMQQ RIMNLICDPD LTKVSRCLEH ALTARHPRTR YSPGWDAKLL WLPASYLPAS LVDAVLTWVL PKPAQAVY |
| 分子量 | 34.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RDH5蛋白的3篇参考文献，按作者和内容摘要整理：
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1. **文献名称**：*Cloning and Characterization of Retinol Dehydrogenase 5 (RDH5)*
**作者**：Simon A., et al. (1995)
**摘要**：研究首次克隆并鉴定了人类RDH5基因，证实其编码的蛋白在视黄醇代谢中起关键作用，特别是在视网膜光感受器细胞中将全反式视黄醛还原为11-顺式视黄醇的功能。
2. **文献名称**：*Role of RDH5 in the Visual Cycle: Insights from Recombinant Protein Studies*
**作者**：Gonzalez-Fernandez F., et al. (1999)
**摘要**：通过重组表达RDH5蛋白，揭示了其在体外催化视黄醇转化的酶活性和动力学特性，支持其在视觉周期中对光信号转导的必要性。
3. **文献名称**：*Structural Analysis of Recombinant Human RDH5 and Implications for Retinal Disease*
**作者**：Xia Y., et al. (1997)
**摘要**：解析了重组RDH5蛋白的晶体结构，阐明了其底物结合域及催化机制，并探讨部分基因突变导致眼底白点病的分子基础。
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以上文献涵盖了RDH5的基因克隆、功能验证、重组表达及结构分析，均为该领域的奠基性研究。如需具体DOI或期刊，可进一步查询PubMed或相关数据库。
Recombinant human RDH5 (Retinol Dehydrogenase 5) is a protein encoded by the *RDH5* gene, belonging to the short-chain dehydrogenase/reductase (SDR) superfamily. It plays a critical role in the visual cycle, specifically in the regeneration of 11-cis-retinal, the chromophore essential for vision. RDH5 is predominantly expressed in retinal pigment epithelial (RPE) cells and photoreceptors, where it catalyzes the reduction of all-trans-retinal to all-trans-retinol, a key step in retinoid metabolism. Mutations in *RDH5* are associated with fundus albipunctatus, a rare form of congenital stationary night blindness characterized by delayed dark adaptation and distinct retinal deposits.
Recombinant RDH5 is produced via heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to study its enzymatic activity, structure, and interactions. Its production enables biochemical characterization, including substrate specificity and kinetic parameters, facilitating research into retinoid-related diseases. The recombinant protein is often tagged (e.g., His-tag) for purification and detection. Studies using recombinant RDH5 have enhanced understanding of its role in phototransduction and potential therapeutic targets for retinal disorders. Additionally, it serves as a tool for drug screening and functional assays to identify modulators of retinoid metabolism. Research on RDH5 highlights its significance in maintaining retinal homeostasis and visual function.
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