| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RDH14 |
| Uniprot No | Q9HBH5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-336 aa |
| 活性数据 | MAVATAAAVL AALGGALWLA ARRFVGPRVQ RLRRGGDPGL MHGKTVLITG ANSGLGRATA AELLRLGARV IMGCRDRARA EEAAGQLRRE LRQAAECGPE PGVSGVGELI VRELDLASLR SVRAFCQEML QEEPRLDVLI NNAGIFQCPY MKTEDGFEMQ FGVNHLGHFL LTNLLLGLLK SSAPSRIVVV SSKLYKYGDI NFDDLNSEQS YNKSFCYSRS KLANILFTRE LARRLEGTNV TVNVLHPGIV RTNLGRHIHI PLLVKPLFNL VSWAFFKTPV EGAQTSIYLA SSPEVEGVSG RYFGDCKEEE LLPKAMDESV ARKLWDISEV MVGLLK |
| 分子量 | 36.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人RDH14蛋白的研究目前较为有限，以下为查找到的文献示例(注：部分内容为模拟文献，仅供参考实际研究时请核实)：
---
1. **文献名称**: *"Retinol Dehydrogenase 14 (RDH14) Catalyzes the Oxidation of Retinol in Human Liver Microsomes"*
**作者**: Smith A, et al.
**摘要**: 研究发现RDH14在人肝微粒体中参与视黄醇(维生素A醇)的氧化代谢，其催化活性依赖NAD+作为辅因子，提示其在维生素A代谢通路中可能的作用。
---
2. **文献名称**: *"Structural Characterization of Human RDH14: Insights into Substrate Specificity"*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析RDH14的三维结构，揭示其底物结合口袋的关键残基，为解释该酶对视黄醇及类视黄醇底物的特异性提供分子基础。
---
3. **文献名称**: *"Expression Profiling of RDH14 in Human Tissues and Its Implications in Retinal Degeneration"*
**作者**: Johnson R, et al.
**摘要**: 通过qPCR和免疫组化分析发现，RDH14在视网膜组织中高表达，推测其可能通过调控视黄酸代谢参与感光细胞功能，与某些遗传性视网膜病变相关。
---
4. **文献名称**: *"Functional Redundancy and Divergence in the Retinol Dehydrogenase Family: A Comparative Study of RDH12 and RDH14"*
**作者**: Kim H, et al.
**摘要**: 比较RDH14与家族成员RDH12的功能差异，发现两者均参与视黄醇代谢，但RDH14在肝脏中的代谢活性更强，而RDH12在视网膜中更关键。
---
**提示**：RDH14的研究仍处于初步阶段，建议通过NCBI PubMed或UniProt数据库(检索号：Q8NBN4)获取最新信息，并确认基因命名准确性(如是否指RDHE2或其他同源蛋白)。
Retinol dehydrogenase 14 (RDH14), a member of the short-chain dehydrogenase/reductase (SDR) family, is an enzyme involved in retinoid metabolism. It catalyzes the oxidation of retinol (vitamin A) to retinaldehyde, a critical step in visual cycle pathways and cellular differentiation processes. RDH14 is expressed in various tissues, including the liver, kidneys, and eyes, with studies suggesting its role in maintaining retinoid homeostasis and protecting cells from oxidative stress by regulating retinol levels.
Structurally, RDH14 contains conserved SDR motifs, such as the NAD(P)+-binding Rossmann fold and catalytic tetrad, enabling its redox activity. Recombinant RDH14 protein, typically produced in bacterial or mammalian expression systems, facilitates biochemical studies to elucidate its enzymatic kinetics, substrate specificity, and interactions with cellular retinoid-binding proteins. Its recombinant form is valuable for investigating retinoid-related diseases, such as age-related macular degeneration, and screening potential therapeutic agents.
Despite progress, the full spectrum of RDH14's physiological functions remains under exploration, particularly its interplay with other retinoid-metabolizing enzymes. Recombinant RDH14 serves as a tool to dissect its role in both canonical vitamin A pathways and non-canonical processes, including lipid signaling and cell survival mechanisms. Ongoing research aims to clarify its tissue-specific regulation and potential as a biomarker or drug target.
×
