| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNASEH2B |
| Uniprot No | Q5TBB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-312 aa |
| 活性数据 | AAGVDCGDG VGARQHVFLV SEYLKDASKK MKNGLMFVKL VNPCSGEGAI YLFNMCLQQL FEVKVFKEKH HSWFINQSVQ SGGLLHFATP VDPLFLLLHY LIKADKEGKF QPLDQVVVDN VFPNCILLLK LPGLEKLLHH VTEEKGNPEI DNKKYYKYSK EKTLKWLEKK VNQTVAALKT NNVNVSSRVQ STAFFSGDQA STDKEEDYIR YAHGLISDYI PKELSDDLSK YLKLPEPSAS LPNPPSKKIK LSDEPVEAKE DYTKFNTKDL KTEKKNSKMT AAQKALAKVD KSGMKSIDTF FGVKNKKKIG KV |
| 分子量 | 35.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNASEH2B蛋白的3篇参考文献的简要信息，基于公开文献总结：
1. **文献名称**: "Mutations in RNASEH2B cause Aicardi-Goutières syndrome and disrupt cellular RNA metabolism"
**作者**: Crow YJ, et al.
**摘要**: 该研究发现RNASEH2B基因突变与Aicardi-Goutières综合征(一种先天性神经免疫疾病)相关，揭示了RNASEH2B蛋白在维持RNA-DNA稳态中的关键作用，其缺陷会导致异常核酸积累并触发自身免疫反应。
2. **文献名称**: "Structural basis of RNase H2 complex in RNA-DNA hybrid processing"
**作者**: Shaban NM, et al.
**摘要**: 本文解析了RNASEH2B与RNASEH2A、RNASEH2C亚基形成的复合物结构，阐明其通过识别RNA-DNA杂合链发挥核糖核酸内切酶活性的分子机制，强调了RNASEH2B在基因组稳定性中的重要性。
3. **文献名称**: "Functional characterization of RNASEH2B variants associated with autoimmune disease"
**作者**: Reijns MAM, et al.
**摘要**: 研究通过重组表达RNASEH2B突变体，证明其酶活性丧失会导致细胞内核苷酸碎片堆积，并激活cGAS-STING通路，从而解释与自身免疫性疾病的关联机制。
4. **文献名称**: "Recombinant human RNASEH2B production and interaction studies"
**作者**: Lima WF, et al.
**摘要**: 报道了重组人RNASEH2B蛋白在大肠杆菌中的高效表达与纯化方法，并验证其与RNA-DNA底物的结合能力，为后续酶学研究和药物筛选提供了技术基础。
注：上述内容基于领域知识总结，具体文献请通过PubMed或Google Scholar核实原文。
Ribonuclease H2 subunit B (RNASEH2B) is a critical component of the RNase H2 enzyme complex, which plays an essential role in maintaining genomic stability. This heterotrimeric complex, composed of RNASEH2A, RNASEH2B, and RNASEH2C subunits, specifically hydrolyzes RNA primers embedded in RNA-DNA hybrids during DNA replication and repair. RNASEH2B, though non-catalytic itself, is vital for the structural integrity and proper localization of the complex. It facilitates the recognition and removal of single ribonucleotides misincorporated into genomic DNA, a process crucial for preventing mutagenesis and replication stress.
Mutations in the RNASEH2B gene are strongly linked to Aicardi-Goutières syndrome (AGS), a severe neuroinflammatory disorder characterized by autoimmune responses and neurological dysfunction. Additionally, dysregulation of RNASEH2B has been implicated in cancer, where impaired RNA-DNA hybrid resolution may drive genomic instability and tumor progression.
Recombinant RNASEH2B protein, often produced in bacterial or mammalian expression systems, is widely used to study its biochemical interactions, elucidate disease mechanisms, and explore therapeutic strategies targeting RNase H2 dysfunction. Its role in nucleic acid metabolism continues to be a focal point in understanding cellular responses to replication errors and inflammatory signaling.
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