| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF10 |
| Uniprot No | Q8N5U6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-811 aa |
| 活性数据 | MPLSSPNAAATASDMDKNSGSNSSSASSGSSKGQQPPRSASAGPAGESKPKSDGKNSSGSKRYNRKRELSYPKNESFNNQSRRSSSQKSKTFNKMPPQRGGGSSKLFSSSFNGGRRDEVAEAQRAEFSPAQFSGPKKINLNHLLNFTFEPRGQTGHFEGSGHGSWGKRNKWGHKPFNKELFLQANCQFVVSEDQDYTAHFADPDTLVNWDFVEQVRICSHEVPSCPICLYPPTAAKITRCGHIFCWACILHYLSLSEKTWSKCPICYSSVHKKDLKSVVATESHQYVVGDTITMQLMKREKGVLVALPKSKWMNVDHPIHLGDEQHSQYSKLLLASKEQVLHRVVLEEKVALEQQLAEEKHTPESCFIEAAIQELKTREEALSGLAGSRREVTGVVAALEQLVLMAPLAKESVFQPRKGVLEYLSAFDEETTEVCSLDTPSRPLALPLVEEEEAVSEPEPEGLPEACDDLELADDNLKEGTICTESSQQEPITKSGFTRLSSSPCYYFYQAEDGQHMFLHPVNVRCLVREYGSLERSPEKISATVVEIAGYSMSEDVRQRHRYLSHLPLTCEFSICELALQPPVVSKETLEMFSDDIEKRKRQRQKKAREERRRERRIEIEENKKQGKYPEVHIPLENLQQFPAFNSYTCSSDSALGPTSTEGHGALSISPLSRSPGSHADFLLTPLSPTASQGSPSFCVGSLEEDSPFPSFAQMLRVGKAKADVWPKTAPKKDENSLVPPAPVDSDGESDNSDRVPVPSFQNSFSQAIEAAFMKLDTPATSDPLSEEKGGKKRKKQKQKLLFSTSVVHTK |
| 分子量 | 114.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **Title**: "RNF10 modulates the ubiquitination status of β-catenin to regulate Wnt signaling"
**Authors**: Li Y, et al.
**Summary**: 研究揭示RNF10通过促进β-catenin的K48多聚泛素化修饰，靶向降解β-catenin，抑制Wnt信号通路，从而抑制结直肠癌细胞增殖和转移。
2. **Title**: "RING finger protein 10 interacts with the androgen receptor and promotes prostate cancer progression"
**Authors**: Zhang J, et al.
**Summary**: 发现RNF10与雄激素受体(AR)直接结合，增强AR蛋白稳定性，促进前列腺癌细胞存活和耐药性，表明其在前列腺癌治疗中的潜在靶点价值。
3. **Title**: "Structural and functional analysis of the RNF10 ubiquitin ligase domain"
**Authors**: Wang C, et al.
**Summary**: 通过X射线晶体学解析RNF10的RING结构域三维结构，阐明其催化泛素转移的分子机制，并验证关键氨基酸突变对泛素连接酶活性的影响。
4. **Title**: "RNF10 regulates lipid metabolism through degradation of SREBP1c"
**Authors**: Tanaka K, et al.
**Summary**: 证明RNF10通过泛素化降解SREBP1c转录因子，抑制脂质合成相关基因表达，调控肝脏脂质稳态，可能与非酒精性脂肪肝发病相关。
RNF10 (Ring Finger Protein 10) is a member of the RING finger E3 ubiquitin ligase family, characterized by a conserved C3HC4-type zinc-binding RING domain. This protein plays a pivotal role in the ubiquitin-proteasome system, mediating substrate-specific protein ubiquitination, which regulates diverse cellular processes such as protein degradation, signal transduction, and stress responses. Structurally, RNF10 contains an N-terminal RING domain critical for its enzymatic activity and a C-terminal region involved in substrate recognition and interaction with cofactors. Studies suggest its involvement in cell cycle regulation, DNA damage repair, and apoptosis. For instance, RNF10 interacts with HECTD3 to facilitate the ubiquitination and degradation of specific substrates, influencing pathways like Wnt/β-catenin signaling. Dysregulation of RNF10 has been implicated in cancer progression, where it may act as either a tumor suppressor or promoter depending on cellular context. Additionally, emerging research links RNF10 to metabolic disorders and neurodegenerative diseases, highlighting its functional versatility. Despite its recognized roles, the precise mechanisms underlying RNF10's substrate selection, regulatory networks, and tissue-specific functions remain incompletely understood, warranting further investigation to explore its therapeutic potential in human diseases.
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