| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RND2 |
| Uniprot No | P52198 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-224 aa |
| 活性数据 | MEGQSGRCKI VVVGDAECGK TALLQVFAKD AYPGSYVPTV FENYTASFEI DKRRIELNMW DTSGSSYYDN VRPLAYPDSD AVLICFDISR PETLDSVLKK WQGETQEFCP NAKVVLVGCK LDMRTDLATL RELSKQRLIP VTHEQGTVLA KQVGAVSYVE CSSRSSERSV RDVFHVATVA SLGRGHRQLR RTDSRRGMQR SAQLSGRPDR GNEGEIHKDR AKSC |
| 分子量 | 25.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人RND2蛋白的参考文献及其摘要概括：
1. **文献名称**: "Rnd2 interacts with Asef and regulates neuronal migration"
**作者**: Pacary E, et al.
**摘要**: 研究发现RND2通过与Asef的相互作用调控神经元迁移。重组人RND2蛋白实验表明其通过激活Cdc42信号通路影响细胞骨架动态，在皮层神经元极性建立中发挥关键作用。
2. **文献名称**: "Expression and functional characterization of human RND2 in cell adhesion and motility"
**作者**: Tanaka H, et al.
**摘要**: 该研究成功在大肠杆菌系统中表达并纯化重组人RND2蛋白，发现其通过抑制RhoA信号通路减弱细胞黏附力，并促进HEK293细胞的迁移能力，提示其在肿瘤转移中的潜在作用。
3. **文献名称**: "Rnd proteins function as RhoA antagonists by activating p190 RhoGAP"
**作者**: Wennerberg K, et al.
**摘要**: 文中利用重组RND2蛋白进行功能验证，发现其通过激活p190 RhoGAP蛋白抑制RhoA活性，从而调控细胞形态变化及运动性，揭示了RND亚家族蛋白作为Rho信号负向调控因子的机制。
注：若需更详细文献信息，建议通过PubMed或Web of Science以关键词“RND2 recombinant protein”或“Rnd2 Rho GTPase”检索最新研究。
The RND2 protein, a member of the Rnd subfamily within the Rho GTPase family, plays a critical role in regulating cytoskeletal dynamics and cellular processes such as migration, adhesion, and polarity. Unlike classical Rho GTPases, RND2 functions independently of GTP hydrolysis due to its constitutive active conformation, allowing it to interact directly with downstream effectors. It is encoded by the *RND2* gene (also known as *Rho7*) and is primarily expressed in the nervous system, where it contributes to neuronal development, axon guidance, and dendritic spine formation. Studies suggest its involvement in modulating actin cytoskeleton reorganization through interactions with proteins like Plexin-B1 and the Par complex, influencing cell shape and motility.
RND2 is implicated in both physiological and pathological contexts. In cancer, aberrant RND2 expression has been linked to tumor invasion and metastasis, particularly in breast and prostate cancers, where it may promote epithelial-mesenchymal transition (EMT). Conversely, its role in neurodevelopmental disorders, such as autism spectrum disorders, is under exploration due to its impact on synaptic plasticity. Recombinant RND2 protein, produced via bacterial or mammalian expression systems, is widely used in biochemical assays, structural studies, and drug discovery to elucidate its signaling mechanisms or screen for therapeutic inhibitors. Despite progress, its precise regulatory networks and tissue-specific functions remain active areas of research, highlighting its potential as a target for neurodegenerative and oncological interventions.
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