| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF122 |
| Uniprot No | Q9H9V4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-155 aa |
| 活性数据 | MHPFQWCNGCFCGLGLVSTNKSCSMPPISFQDLPLNIYMVIFGTGIFVFMLSLIFCCYFISKLRNQAQSERYGYKEVVLKGDAKKLQLYGQTCAVCLEDFKGKDELGVLPCQHAFHRKCLVKWLEVRCVCPMCNKPIASPSEATQNIGILLDELV |
| 分子量 | 43.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人RNF122蛋白的参考文献及简要摘要：
1. **"RNF122 functions as an E3 ubiquitin ligase in ubiquitin-mediated protein degradation"**
- 作者：Zhang Y, et al.
- 摘要：研究揭示了RNF122作为RING型E3泛素连接酶，通过泛素-蛋白酶体途径调控靶蛋白降解，并证实其在神经元发育中的潜在作用。
2. **"RNF122 promotes glioma progression by stabilizing EGFR signaling in glioblastoma"**
- 作者：Li H, et al.
- 摘要：研究发现RNF122在胶质母细胞瘤中高表达，通过泛素化修饰EGFR增强其稳定性，进而促进肿瘤细胞增殖和侵袭。
3. **"Structural analysis of the RNF122 RING domain reveals its interaction with VPS34 in autophagy regulation"**
- 作者：Wang X, et al.
- 摘要：通过晶体结构解析，阐明了RNF122的RING结构域与自噬相关蛋白VPS34的相互作用机制，提示其可能参与自噬调控通路。
4. **"RNF122 suppresses hepatocellular carcinoma by targeting oncogenic β-catenin for ubiquitination"**
- 作者：Chen L, et al.
- 摘要：研究证明RNF122通过泛素化降解β-catenin抑制肝癌细胞生长，揭示了其作为抑癌因子的潜在临床意义。
(注：上述文献为示例性内容，具体研究请以实际发表的论文为准。)
Recombinant human RNF122 (RING Finger Protein 122) is a ubiquitin-protein ligase (E3) belonging to the RING finger family, which plays a crucial role in the ubiquitination process by tagging specific substrates for proteasomal degradation. The protein contains a conserved RING domain at its N-terminus, essential for E3 ligase activity, and a putative transmembrane domain, suggesting potential localization to intracellular membranes like the endoplasmic reticulum. RNF122 is involved in regulating cellular processes such as apoptosis, proliferation, and protein quality control. Studies highlight its interaction with proteins linked to neurodegenerative diseases, including Parkinson’s and Alzheimer’s, by modulating pathogenic protein aggregation. Additionally, it influences neuronal development and synaptic function. Dysregulation of RNF122 has been implicated in cancers, particularly glioblastoma, where it may promote tumor progression by degrading tumor suppressors like PTEN. Recombinant RNF122 is widely used in vitro to study substrate specificity, enzymatic mechanisms, and functional pathways. Its recombinant form, typically expressed in E. coli or mammalian systems, enables biochemical characterization and high-throughput screening for therapeutic agents targeting ubiquitination-related disorders. Emerging evidence suggests RNF122’s role in endoplasmic reticulum stress responses, positioning it as a potential biomarker or therapeutic target for neurodegenerative and malignant diseases. Research remains ongoing to fully elucidate its physiological and pathological networks.
×
