| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF12 |
| Uniprot No | Q9NVW2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-624 aa |
| 活性数据 | MENSDSNDKG SGDQSAAQRR SQMDRLDREE AFYQFVNNLS EEDYRLMRDN NLLGTPGEST EEELLRRLQQ IKEGPPPQNS DENRGGDSSD DVSNGDSIID WLNSVRQTGN TTRSGQRGNQ SWRAVSRTNP NSGDFRFSLE INVNRNNGSQ NSENENEPSA RRSSGENVEN NSQRQVENPR SESTSARPSR SERNSTEALT EVPPTRGQRR ARSRSPDHRR TRARAERSRS PLHPMSEIPR RSHHSISSQT FEHPLVNETE GSSRTRHHVT LRQQISGPEL LSRGLFAASG TRNASQGAGS SDTAASGEST GSGQRPPTIV LDLQVRRVRP GEYRQRDSIA SRTRSRSQTP NNTVTYESER GGFRRTFSRS ERAGVRTYVS TIRIPIRRIL NTGLSETTSV AIQTMLRQIM TGFGELSYFM YSDSDSEPTG SVSNRNMERA ESRSGRGGSG GGSSSGSSSS SSSSSSSSSS SSSSSSPSSS SGGESSETSS DLFEGSNEGS SSSGSSGARR EGRHRAPVTF DESGSLPFLS LAQFFLLNED DDDQPRGLTK EQIDNLAMRS FGENDALKTC SVCITEYTEG NKLRKLPCSH EYHVHCIDRW LSENSTCPIC RRAVLASGNR ESVV |
| 分子量 | 68.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNF12蛋白的3条参考文献及其摘要概括：
1. **文献名称**：*RNF12 is a ubiquitin ligase for the X-chromosomal gene dosage regulator REX1*
**作者**：Gontan, C., et al.
**摘要**：研究发现RNF12作为E3泛素连接酶，通过泛素化降解转录因子REX1.调控X染色体失活的关键启动过程，解释了其在维持X染色体剂量平衡中的作用机制。
2. **文献名称**：*RNF12 activates Xist and is essential for X chromosome inactivation*
**作者**：Barakat, T.S., et al.
**摘要**：该文献揭示RNF12通过激活Xist基因(X失活中心的核心非编码RNA)的表达，在胚胎干细胞中启动X染色体失活，强调了其在哺乳动物性别决定中的核心功能。
3. **文献名称**：*Mutations in RNF12 cause an X-linked intellectual disability syndrome*
**作者**：Frints, S.G.M., et al.
**摘要**：研究鉴定了RNF12基因突变与X连锁智力障碍综合征的关联，表明其泛素连接酶活性异常可能导致神经发育缺陷，拓展了RNF12在人类疾病中的临床意义。
以上内容涵盖RNF12的分子机制、X染色体调控及疾病相关性研究方向。
**Background of Recombinant Human RNF12 Protein**
Recombinant human RNF12 (RING finger protein 12), also known as RLIM (RING finger LIM domain-binding protein), is an E3 ubiquitin ligase encoded by the *RLIM* gene. As a member of the RING finger protein family, RNF12 contains a characteristic RING domain critical for its enzymatic activity in mediating substrate ubiquitination, a process essential for protein degradation, trafficking, or functional modulation via the ubiquitin-proteasome system. RNF12 plays multifaceted roles in cellular processes, including X-chromosome inactivation (XCI), embryonic development, and signal transduction. Notably, it interacts with key regulators like the pluripotency factor REX1. targeting them for degradation to ensure proper cell differentiation and developmental timing.
Dysregulation of RNF12 is linked to human diseases, particularly X-linked intellectual disability syndromes (XLID) and certain cancers, underscoring its importance in neural and developmental pathways. Recombinant RNF12 proteins, produced in heterologous expression systems (e.g., *E. coli* or mammalian cells), retain functional integrity and are widely used to study molecular mechanisms of ubiquitination, XCI, and disease pathology. These recombinant tools enable biochemical assays, structural analyses, and therapeutic exploration, such as identifying inhibitors or modulators of RNF12 activity. Ongoing research continues to unravel its interactions with substrates and its potential as a biomarker or therapeutic target in genetic and oncological disorders.
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