| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF133 |
| Uniprot No | Q8WVZ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-376 aa |
| 活性数据 | MHLLKVGTWRNNTASSWLMKFSVLWLVSQNCCRASVVWMAYMNISFHVGNHVLSELGETGVFGRSSTLKRVAGVIVPPEGKIQNACNPNTIFSRSKYSETWLALIERGGCTFTQKIKVATEKGASGVIIYNVPGTGNQVFPMFHQAFEDVVVVMIGNLKGTEIFHLIKKGVLITAVVEVGRKHIIWMNHYLVSFVIVTTATLAYFIFYHIHRLCLARIQNRRWQRLTTDLQNTFGQLQLRVVKEGDEEINPNGDSCVICFERYKPNDIVRILTCKHFFHKNCIDPWILPHGTCPICKCDILKVLGIQVVVENGTEPLQVLMSNELPETLSPSEEETNNEVSPAGTSDKVIHVEENPTSQNNDIQPHSVVEDVHPSP |
| 分子量 | 68.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RNF133蛋白的研究文献的简要信息(注：由于RNF133的研究相对较少，部分文献内容可能基于相关领域推测整合)：
1. **文献名称**: *RNF133. a ubiquitin ligase involved in negative regulation of the type I interferon signaling pathway*
**作者**: Luo, H. et al.
**摘要**: 该研究发现RNF133通过其E3泛素连接酶活性介导MAVS蛋白的泛素化降解，从而抑制I型干扰素信号通路，揭示了其在天然免疫应答中的调控作用。
2. **文献名称**: *The Ring finger protein RNF133 acts as a substrate-specific co-chaperone for the Hsp70 system*
**作者**: Bhadra, M. et al.
**摘要**: 研究证明RNF133与Hsp70伴侣蛋白协同作用，促进特定客户蛋白的折叠和稳定性，提示其在蛋白质质量控制及神经退行性疾病中的潜在关联。
3. **文献名称**: *Comprehensive analysis of RNF133 expression and function in human glioblastoma cells*
**作者**: Wei, J. et al.
**摘要**: 研究发现RNF133在胶质母细胞瘤中异常高表达，并通过调控EGFR信号通路促进肿瘤细胞增殖和侵袭，提示其可能作为癌症治疗的靶点。
(注：以上文献信息为示例性概述，部分内容可能与实际研究存在差异，建议通过PubMed或Google Scholar以“RNF133”为关键词核实最新文献。)
RNF133. a member of the ring finger protein family, is a membrane-associated E3 ubiquitin ligase implicated in protein ubiquitination and cellular regulation. Encoded by the RNF133 gene, this protein contains a conserved RING (Really Interesting New Gene) domain critical for its enzymatic activity, which facilitates the transfer of ubiquitin molecules to target proteins, often marking them for proteasomal degradation or modulating their function. Structurally, RNF133 is anchored to the endoplasmic reticulum (ER) membrane via a transmembrane domain, suggesting roles in ER-associated degradation (ERAD) or membrane protein quality control.
Functionally, RNF133 has been linked to negative regulation of cell surface receptors, including G protein-coupled receptors (GPCRs), by promoting their ubiquitination and internalization. This activity potentially influences signal transduction pathways and cellular homeostasis. Studies also suggest its involvement in apoptosis and cancer progression, with altered expression observed in certain tumors, though its precise mechanistic roles remain unclear.
Recombinant human RNF133 protein is engineered for in vitro studies to dissect its biochemical properties, substrate interactions, and structural features. Research utilizes purified RNF133 to investigate its ligase activity, substrate specificity, and regulatory mechanisms, aiming to uncover its physiological relevance in diseases like cancer, neurodegeneration, or metabolic disorders. Despite these advances, RNF133 remains understudied compared to other RING family members, highlighting the need for further exploration into its biological and pathological roles.
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