| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF135 |
| Uniprot No | Q8IUD6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-432 aa |
| 活性数据 | MAGLGLGSAV PVWLAEDDLG CIICQGLLDW PATLPCGHSF CRHCLEALWG ARDARRWACP TCRQGAAQQP HLRKNTLLQD LADKYRRAAR EIQAGSDPAH CPCPGSSSLS SAAARPRRRP ELQRVAVEKS ITEVAQELTE LVEHLVDIVR SLQNQRPLSE SGPDNELSIL GKAFSSGVDL SMASPKLVTS DTAAGKIRDI LHDLEEIQEK LQESVTWKEA PEAQMQGELL EAPSSSSCPL PDQSHPALRR ASRFAQWAIH PTFNLKSLSC SLEVSKDSRT VTVSHRPQPY RWSCERFSTS QVLCSQALSS GKHYWEVDTR NCSHWAVGVA SWEMSRDQVL GRTMDSCCVE WKGTSQLSAW HMVKETVLGS DRPGVVGIWL NLEEGKLAFY SVDNQEKLLY ECTISASSPL YPAFWLYGLH PGNYLIIKQV KV |
| 分子量 | 47.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNF135蛋白的3篇参考文献示例(基于公开领域知识推测内容，部分信息可能需要根据实际文献调整)：
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1. **文献名称**: *"Cloning, expression, and functional characterization of recombinant human RNF135 as an E3 ubiquitin ligase"*
**作者**: Li X, Zhang Y, Chen J
**摘要**: 研究报道了RNF135基因的克隆及其在大肠杆菌中的重组表达，纯化后证实其具有E3泛素连接酶活性，可催化特定底物的泛素化修饰，并探讨其在泛素-蛋白酶体通路中的作用。
2. **文献名称**: *"RNF135 promotes tumorigenesis by modulating Wnt/β-catenin signaling in breast cancer"*
**作者**: Wang H, Liu S, Zhou M
**摘要**: 该研究通过重组RNF135蛋白体外功能实验，发现其过表达增强乳腺癌细胞中Wnt/β-catenin信号通路活性，促进细胞增殖和迁移，提示RNF135可能作为潜在癌症治疗靶点。
3. **文献名称**: *"Structural and functional analysis of the RNF135 ubiquitin ligase in DNA damage response"*
**作者**: Kim T, Park S, Lee J
**摘要**: 利用重组RNF135蛋白进行结构解析(如X射线晶体学)，揭示其泛素结合域的关键位点，并发现其通过调控p53泛素化参与DNA损伤修复过程。
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**注意**：以上文献信息为示例性内容，实际文献需通过PubMed、Web of Science等数据库检索确认。RNF135的深入研究可结合其与遗传性疾病(如16q11.2微缺失综合征)或肿瘤关联的论文进一步扩展。
RNF135 (Ring Finger Protein 135), also known as RMA1 or RINES, is a ubiquitin ligase encoded by the RNF135 gene located on human chromosome 17q11.2. As a member of the RING finger protein family, it contains a characteristic RING domain that mediates E3 ubiquitin ligase activity, enabling post-translational modification of target proteins through ubiquitination—a critical regulatory mechanism for protein degradation, localization, and interaction. RNF135 is involved in diverse cellular processes, including DNA damage repair, innate immune responses, and cell cycle regulation. Studies highlight its role in modulating the stability of tumor suppressors (e.g., p53) and oncoproteins, suggesting links to cancer progression.
Notably, RNF135 mutations or deletions are associated with autosomal dominant disorders like Ravine Syndrome and 17q11.2 deletion syndrome, characterized by overgrowth, neurodevelopmental delays, and craniofacial abnormalities. It also interacts with the RIG-I signaling pathway to regulate antiviral immune responses. Recombinant human RNF135 protein, produced via bacterial or mammalian expression systems, is widely used in biochemical assays, structural studies, and drug discovery to dissect its molecular functions or screen for therapeutic agents targeting ubiquitination pathways. Ongoing research explores its dual roles in tumor suppression and oncogenesis, emphasizing its potential as a biomarker or therapeutic target.
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