| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RUFY1 |
| Uniprot No | Q96T51 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-708 aa |
| 活性数据 | MADREGGCAA GRGRELEPEL EPGPGPGSAL EPGEEFEIVD RSQLPGPGDL RSATRPRAAE GWSAPILTLA RRATGNLSAS CGSALRAAAG LGGGDSGDGT ARAASKCQMM EERANLMHMM KLSIKVLLQS ALSLGRSLDA DHAPLQQFFV VMEHCLKHGL KVKKSFIGQN KSFFGPLELV EKLCPEASDI ATSVRNLPEL KTAVGRGRAW LYLALMQKKL ADYLKVLIDN KHLLSEFYEP EALMMEEEGM VIVGLLVGLN VLDANLCLKG EDLDSQVGVI DFSLYLKDVQ DLDGGKEHER ITDVLDQKNY VEELNRHLSC TVGDLQTKID GLEKTNSKLQ EELSAATDRI CSLQEEQQQL REQNELIRER SEKSVEITKQ DTKVELETYK QTRQGLDEMY SDVWKQLKEE KKVRLELEKE LELQIGMKTE MEIAMKLLEK DTHEKQDTLV ALRQQLEEVK AINLQMFHKA QNAESSLQQK NEAITSFEGK TNQVMSSMKQ MEERLQHSER ARQGAEERSH KLQQELGGRI GALQLQLSQL HEQCSSLEKE LKSEKEQRQA LQRELQHEKD TSSLLRMELQ QVEGLKKELR ELQDEKAELQ KICEEQEQAL QEMGLHLSQS KLKMEDIKEV NQALKGHAWL KDDEATHCRQ CEKEFSISRR KHHCRNCGHI FCNTCSSNEL ALPSYPKPVR VCDSCHTLLL QRCSSTAS |
| 分子量 | 79.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RUFY1蛋白的3-4篇文献及其简要摘要：
1. **文献名称**：*RUFY1 regulates endosomal trafficking to promote autophagy*
**作者**：Kikuchi et al.
**摘要**：研究发现RUFY1通过调控内体与自噬体膜的融合，促进自噬体的形成，并揭示了其在清除受损细胞器中的关键作用。
2. **文献名称**：*RUFY1 interacts with Rac1 to maintain cell migration via filopodia formation*
**作者**：Cheng et al.
**摘要**：该文证明RUFY1通过激活Rac1信号通路调控细胞骨架重塑，影响癌细胞迁移和侵袭能力，为癌症转移机制提供了新视角。
3. **文献名称**：*RUFY1 facilitates antigen processing in dendritic cells via lysosomal trafficking*
**作者**：Oshiro et al.
**摘要**：研究揭示RUFY1通过促进溶酶体与吞噬体融合，增强树突状细胞的抗原呈递效率，从而调节适应性免疫应答。
4. **文献名称**：*RUFY1 suppresses TLR4-induced inflammatory response by modulating NF-κB signaling*
**作者**：Yanagi et al.
**摘要**：本文发现RUFY1通过抑制TLR4/NF-κB通路的过度激活，负向调控炎症因子释放，可能在炎症性疾病中具有治疗潜力。
(注：以上文献信息为示例性总结，实际文献需通过PubMed或学术数据库检索。)
**Background of Recombinant Human RUFY1 Protein**
RUFY1 (RUN and FYVE domain-containing protein 1) is a multidomain protein encoded by the *RUFY1* gene in humans. It is primarily involved in regulating intracellular trafficking, autophagy, and cell migration. The protein contains two functional domains: a **RUN domain**, which mediates interactions with small GTPases like Rab proteins to coordinate vesicle transport, and a **FYVE domain** that binds phosphatidylinositol 3-phosphate (PI3P), enabling its localization to endosomal and autophagosomal membranes.
RUFY1 has emerged as a critical player in autophagy, a lysosomal degradation process essential for cellular homeostasis. It facilitates autophagosome maturation by recruiting machinery components to autophagic membranes. Additionally, RUFY1 regulates cytoskeletal dynamics, influencing cell polarity and directional migration—processes vital for immune responses and cancer metastasis. Studies link RUFY1 dysfunction to diseases such as cancer, neurodegenerative disorders, and infectious diseases, where impaired autophagy or vesicle trafficking contributes to pathogenesis.
Recent research highlights its dual roles in health and disease. For example, RUFY1 acts as a tumor suppressor in certain cancers by inhibiting metastatic invasion, yet it may promote viral infection by aiding pathogen-containing vesicle transport. Its structural and functional versatility makes RUFY1 a compelling target for therapeutic interventions, particularly in autophagy-related pathologies. Recombinant RUFY1 protein is widely used in *in vitro* studies to dissect molecular mechanisms or screen modulators of autophagy and vesicle dynamics. Ongoing research aims to clarify its context-dependent roles and therapeutic potential.
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