| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAMD11 |
| Uniprot No | Q96NU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681 aa |
| 活性数据 | MSKGILQVHP PICDCPGCRI SSPVNRGRLA DKRTVALPAA RNLKKERTPS FSASDGDSDG SGPTCGRRPG LKQEDGPHIR IMKRRVHTHW DVNISFREAS CSQDGNLPTL ISSVHRSRHL VMPEHQSRCE FQRGSLEIGL RPAGDLLGKR LGRSPRISSD CFSEKRARSE SPQEALLLPR ELGPSMAPED HYRRLVSALS EASTFEDPQR LYHLGLPSHG EDPPWHDPPH HLPSHDLLRV RQEVAAAALR GPSGLEAHLP SSTAGQRRKQ GLAQHREGAA PAAAPSFSER ELPQPPPLLS PQNAPHVALG PHLRPPFLGV PSALCQTPGY GFLPPAQAEM FAWQQELLRK QNLARLELPA DLLRQKELES ARPQLLAPET ALRPNDGAEE LQRRGALLVL NHGAAPLLAL PPQGPPGSGP PTPSRDSARR APRKGGPGPA SARPSESKEM TGARLWAQDG SEDEPPKDSD GEDPETAAVG CRGPTPGQAP AGGAGAEGKG LFPGSTLPLG FPYAVSPYFH TGAVGGLSMD GEEAPAPEDV TKWTVDDVCS FVGGLSGCGE YTRVFREQGI DGETLPLLTE EHLLTNMGLK LGPALKIRAQ VARRLGRVFY VASFPVALPL QPPTLRAPER ELGTGEQPLS PTTATSPYGG GHALAGQTSP KQENGTLALL PGAPDPSQPL C |
| 分子量 | 72.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SAMD11蛋白的3篇参考文献范例(注：文献信息为合理虚构示例，实际研究中可能需要根据具体数据库补充核实)：
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1. **文献名称**: *SAMD11 regulates neural differentiation via chromatin remodeling*
**作者**: Li X. et al. (2016), *Nature Cell Biology*
**摘要**: 报道SAMD11蛋白在胚胎干细胞神经分化中的调控作用，通过重组人SAMD11蛋白体外实验，发现其与染色质重塑复合体相互作用，抑制分化关键基因的转录。
2. **文献名称**: *Expression and Structural Analysis of Recombinant SAMD11 in E. coli*
**作者**: Gonzalez R. et al. (2020), *Journal of Biological Chemistry*
**摘要**: 首次实现人源SAMD11蛋白的大肠杆菌重组表达，优化纯化条件后解析其N端 sterile alpha motif (SAM) 结构域，揭示其可能参与蛋白寡聚化。
3. **文献名称**: *SAMD11 interacts with TGF-β signaling components: A yeast two-hybrid screening*
**作者**: Wang Y. et al. (2018), *Cell Reports*
**摘要**: 利用重组SAMD11蛋白作为诱饵进行酵母双杂交筛选，发现其与TGF-β通路中的SMAD4存在直接结合，提示其在细胞信号转导中的潜在作用。
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**注意**：SAMD11相关研究仍较少，实际文献可能集中在基因组关联分析(如眼部疾病或癌症)，关于重组蛋白的细节研究建议结合UniProt(SAMD11)或PubMed最新文献筛选。
SAMD11 (Sterile Alpha Motif Domain-containing 11) is a nuclear protein encoded by the *SAMD11* gene located on chromosome 1p36.33. It belongs to the SAM domain-containing protein family, known for mediating protein-protein interactions and participating in transcriptional regulation. SAMD11 contains a conserved SAM domain, which likely facilitates molecular interactions, though its precise functional mechanisms remain under investigation. Studies suggest its involvement in modulating chromatin structure and epigenetic regulation, potentially influencing cell differentiation and development.
Notably, *SAMD11* lies within a genomic region associated with age-related macular degeneration (AMD). Its co-expression with neighboring genes, such as *NRBP2*, hints at functional synergies in retinal homeostasis. SAMD11 has also been linked to disease contexts, including altered expression in lung adenocarcinoma and neurodevelopmental disorders, though its role in these pathologies requires further validation.
Recombinant human SAMD11 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for biochemical and structural studies. Purification typically involves affinity chromatography and tag-based methods to ensure high yield and purity. This recombinant tool enables exploration of SAMD11's molecular interactions, DNA-binding properties, and regulatory pathways, aiding research into its physiological roles and therapeutic potential. Current challenges include elucidating its specific binding partners and downstream targets across tissue types.
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