| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD1A |
| Uniprot No | P06126 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312aa |
| 氨基酸序列 | MWNWLKEPLSFHVIWIASFYNHSWKQNLVSGWLSDLQTHTWDSNSSTIVF LWPWSRGNFSNEEWKELETLFRIRTIRSFEGIRRYAHELQFEYPFEIQVT GGCELHSGKVSGSFLQLAYQGSDFVSFQNNSWLPYPVAGNMAKHFCKVLN QNQHENDITHNLLSDTCPRFILGLLDAGKAHLQRQVKPEAWLSHGPSPGP GHLQLVCHVSGFYPKPVWVMWMRGEQEQQGTQRGDILPSADGTWYLRATL EVAAGEAADLSCRVKHSSLEGQDIVLYWEHHSSVGFIILAVIVPLLLLIG LALWFRKRCFC |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD1A重组蛋白的参考文献示例(注：以下文献信息为模拟示例，实际引用请核实原文)：
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1. **文献名称**: *"Recombinant CD1A Protein Expression and Functional Characterization in Lipid Antigen Presentation"*
**作者**: Smith A, et al.
**摘要**: 本研究通过在大肠杆菌中重组表达CD1A蛋白，优化了其纯化流程，并验证了其与脂质抗原(如鞘脂类)的结合能力。实验表明，重组CD1A能够有效激活人源T细胞系，支持其在免疫研究中的应用。
2. **文献名称**: *"Structural Basis of CD1A-Mediated Lipid Recognition by T Cells"*
**作者**: Jones B, et al.
**摘要**: 通过X射线晶体学解析了重组CD1A蛋白与脂质抗原复合物的三维结构，揭示了CD1A抗原结合槽的疏水性特征及其与不同脂质分子相互作用的关键残基。
3. **文献名称**: *"High-Yield Production of Soluble CD1A in Mammalian Cells for Immunotherapy Studies"*
**作者**: Lee C, et al.
**摘要**: 在HEK293细胞中实现了CD1A重组蛋白的高效分泌表达，纯化产物在体外成功诱导了特异性T细胞反应，为基于CD1A的疫苗开发提供了技术基础。
4. **文献名称**: *"CD1A-Reactive T Cells in Human Skin Diseases: Insights from Recombinant Protein Assays"*
**作者**: Garcia D, et al.
**摘要**: 利用重组CD1A蛋白检测患者血清中的自身抗体及T细胞应答，发现CD1A在银屑病等皮肤炎症性疾病中可能通过异常脂质抗原呈递参与病理过程。
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**说明**：以上文献为模拟生成内容，实际研究中请通过PubMed、Web of Science等平台检索关键词“CD1A recombinant protein”、“CD1A lipid antigen”等获取准确信息。建议关注领域内权威期刊如*Nature Immunology*、*Journal of Immunology*等。
CD1a is a member of the CD1 family of transmembrane glycoproteins, which play a critical role in the immune system by presenting lipid-based antigens to T cells. Unlike MHC molecules that primarily present peptide antigens, CD1 proteins specialize in binding and displaying lipid, glycolipid, or hydrophobic metabolites to specific T-cell subsets, bridging innate and adaptive immunity. The CD1 family is divided into group 1 (CD1a, CD1b, CD1c, and CD1e) and group 2 (CD1d). CD1a is primarily expressed on dendritic cells, Langerhans cells, and cortical thymocytes, where it facilitates immune surveillance in peripheral tissues and lymphoid organs.
Structurally, CD1a contains three extracellular domains (α1. α2. and α3) and a transmembrane region. The α1 and α2 domains form a hydrophobic antigen-binding groove optimized for accommodating lipid tails, while the α3 domain interacts with β2-microglobulin for stability. Recombinant CD1a proteins are engineered using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper folding, glycosylation, and functional antigen-presenting capabilities. These proteins are pivotal for studying CD1a-mediated immune responses, including its interaction with lipid antigens from pathogens like *Mycobacterium tuberculosis* or self-lipids involved in autoimmune diseases.
Research applications include elucidating mechanisms of lipid antigen presentation, T-cell receptor recognition, and CD1a’s role in diseases such as cancer, infections, and psoriasis. Recombinant CD1a also aids in developing immunotherapies or vaccines targeting lipid-specific immune pathways. Its study has revealed unique features, such as pH-dependent antigen loading in endosomal compartments and distinct trafficking patterns compared to other CD1 isoforms. Despite progress, challenges remain in characterizing the full spectrum of CD1a ligands and their immunological relevance.
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