| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | KNT1 |
| Uniprot No | A0A1Z4NLL6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-88aa |
| 氨基酸序列 | MFLEPKPTQQIDRLNLADQIIQRILTLKEKQVIAIGLYGSLARGTDQLYSDIEIKCILNTEEEDYSWEWIEDGCKIEINFESEDVILN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于KNT1重组蛋白的虚构参考文献示例(仅供学术写作参考框架)：
1. **文献名称**: "Structural and Functional Characterization of Recombinant KNT1 Protein in Neurodegenerative Pathways"
**作者**: Takahashi, S., et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了KNT1重组蛋白，解析了其晶体结构(分辨率为2.1Å)，揭示了其C端结构域在神经元突触可塑性中的关键作用。体外实验证实KNT1与Tau蛋白存在特异性相互作用，可能为阿尔茨海默病机制提供新见解。
2. **文献名称**: "KNT1 Recombinant Protein Enhances Axonal Regeneration in Spinal Cord Injury Models"
**作者**: Chen, L., & Park, H.
**摘要**: 利用哺乳动物细胞表达系统制备了高活性KNT1重组蛋白，通过大鼠脊髓损伤模型证实其能显著促进神经轴突再生(再生率提高47%)。机制研究表明KNT1通过激活PI3K/Akt/mTOR信号通路发挥促修复功能。
3. **文献名称**: "Development of a Novel KNT1-ELISA Diagnostic Kit Based on Recombinant Antigen"
**作者**: Müller, R., et al.
**摘要**: 本研究优化了KNT1重组蛋白在昆虫杆状病毒系统的分泌表达工艺，开发出灵敏度达0.1ng/mL的检测试剂盒。临床验证显示，血清KNT1水平与多发性硬化症患者疾病活动度呈正相关(r=0.82. p<0.001)。
注：以上内容为模拟文献，实际研究中请通过PubMed/Web of Science等平台检索真实文献。建议使用关键词组合如"recombinant KNT1 protein"+"expression"+"function"进行精准检索。
**Background of KNT1 Recombinant Protein**
KNT1 (Kinase Novel Target 1) recombinant protein is a genetically engineered biomolecule designed to facilitate research in cellular signaling and therapeutic development. Derived from its native counterpart, KNT1 is implicated in regulating critical pathways such as cell proliferation, differentiation, and apoptosis. The gene encoding KNT1 is often overexpressed or mutated in certain cancers and inflammatory disorders, making it a focal point for drug discovery.
Recombinant KNT1 is typically produced using expression systems like *E. coli*, yeast, or mammalian cells, ensuring high purity and activity through affinity chromatography. Its recombinant form allows precise control over post-translational modifications, enhancing reproducibility in experimental settings. Researchers utilize KNT1 recombinant protein to study kinase-substrate interactions, screen inhibitory compounds, and elucidate structural features via X-ray crystallography or cryo-EM.
In therapeutic contexts, KNT1 is explored as a biomarker or target for small-molecule inhibitors and monoclonal antibodies. Its role in disease progression, particularly in oncology, has spurred preclinical studies to assess its inhibition efficacy in tumor models. Additionally, recombinant KNT1 supports diagnostic assay development, aiding in patient stratification for precision medicine.
Despite its potential, challenges remain in understanding tissue-specific functions, off-target effects of inhibitors, and resistance mechanisms. Ongoing research aims to map KNT1's interactome and validate its clinical relevance, positioning it as a promising candidate for next-generation therapies.
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