| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCML4 |
| Uniprot No | Q8N228 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-305 aa |
| 活性数据 | MPCQRTAWIGCDRQRTPPFHWREIKSRVLMTPLALSPPRSTPEPDLSSIPQDAATVPSLAAPQALTVCLYINKQANAGPYLERKKVQQLPEHFGPERPSAVLQQAVQACIDCAHQQKLVFSLVKQGYGGEMVSVSASFDGKQHLRSLPVVNSIGYVLRFLAKLCRSLLCDDLFSHQPFPRGCSASEKVQEKEEGRMESVKTVTTEEYLVNPVGMNRYSVDTSASTFNHRGSLHPSSSLYCKRQNSGDSHLGGGPAATAGGPRTSPMSSGGPSAPGLRPPASSPKRNTTSLEGNRCGNVMHASASH |
| 分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于SCML4相关研究的示例性参考文献(注：部分内容为模拟构造，仅供参考，实际文献需通过数据库检索确认)：
1. **文献名称**: "Characterization of Recombinant Human SCML4 as a Novel Polycomb Group Protein Involved in Chromatin Remodeling"
**作者**: Li X, et al.
**摘要**: 本研究首次报道了重组人SCML4蛋白在大肠杆菌中的表达与纯化，验证其作为Polycomb家族成员通过结合H2A泛素化位点参与染色质重塑的功能，并证明其与Ring1B的相互作用。
2. **文献名称**: "SCML4 Regulates DNA Damage Response via Modulating Histone Ubiquitination in Germ Cells"
**作者**: Tanaka K, et al.
**摘要**: 通过昆虫细胞系统表达重组SCML4.发现其通过调控H2AK119泛素化水平影响生殖细胞中DNA损伤修复通路，敲低实验表明SCML4缺失导致减数分裂异常。
3. **文献名称**: "Structural Insights into SCML4-PRC1 Complex Assembly by Cryo-EM"
**作者**: Wang Y, et al.
**摘要**: 利用哺乳细胞表达重组SCML4并与PRC1复合体共纯化，通过冷冻电镜解析复合物结构，揭示SCML4通过N端结构域介导PRC1在特定基因组区域的靶向结合。
4. **文献名称**: "Developmental Stage-Specific Expression of SCML4 in Mouse Testis and Its Role in Spermatogenesis"
**作者**: Chen R, et al.
**摘要**: 研究在小鼠模型中探讨SCML4的表达模式，发现重组人SCML4蛋白体外可挽救生殖干细胞分化缺陷，表明其在精子发生中起关键调控作用。
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**注**：以上文献为示例性质，实际研究中关于SCML4的论文较为有限，建议通过PubMed或Web of Science以“SCML4”或“Polycomb SCML4”为关键词检索最新文献。若需具体论文协助定位，请提供更多研究背景。
**Background of Recombinant Human SCML4 Protein**
SCML4 (Sex Comb on Midleg-like 4) is a member of the polycomb group (PcG) protein family, which plays critical roles in epigenetic regulation and chromatin remodeling. These proteins are essential for maintaining cellular identity, controlling gene expression patterns, and ensuring proper developmental processes. SCML4. specifically, is implicated in reproductive development and spermatogenesis, with studies suggesting its involvement in modulating SUMOylation pathways—a post-translational modification crucial for protein function and stability.
Structurally, SCML4 contains conserved domains such as the SAM (sterile alpha motif) domain, which facilitates protein-protein interactions, and MBT (malignant brain tumor) repeats, known for binding methylated histones. Dysregulation of SCML4 has been linked to male infertility and certain cancers, highlighting its importance in cellular homeostasis.
Recombinant human SCML4 protein, produced via expression systems like *E. coli* or mammalian cells, enables in-depth study of its biochemical properties, interactions, and mechanisms. It serves as a vital tool for exploring SCML4’s role in epigenetic silencing, DNA repair, and germ cell development, offering potential therapeutic insights for related disorders. Research on SCML4 continues to uncover its dual regulatory functions, balancing gene activation and repression in a context-dependent manner.
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