| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEC23B |
| Uniprot No | Q15437 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-767 aa |
| 活性数据 | ATYLEFIQQ NEERDGVRFS WNVWPSSRLE ATRMVVPLAC LLTPLKERPD LPPVQYEPVL CSRPTCKAVL NPLCQVDYRA KLWACNFCFQ RNQFPPAYGG ISEVNQPAEL MPQFSTIEYV IQRGAQSPLI FLYVVDTCLE EDDLQALKES LQMSLSLLPP DALVGLITFG RMVQVHELSC EGISKSYVFR GTKDLTAKQI QDMLGLTKPA MPMQQARPAQ PQEHPFASSR FLQPVHKIDM NLTDLLGELQ RDPWPVTQGK RPLRSTGVAL SIAVGLLEGT FPNTGARIML FTGGPPTQGP GMVVGDELKI PIRSWHDIEK DNARFMKKAT KHYEMLANRT AANGHCIDIY ACALDQTGLL EMKCCANLTG GYMVMGDSFN TSLFKQTFQR IFTKDFNGDF RMAFGATLDV KTSRELKIAG AIGPCVSLNV KGPCVSENEL GVGGTSQWKI CGLDPTSTLG IYFEVVNQHN TPIPQGGRGA IQFVTHYQHS STQRRIRVTT IARNWADVQS QLRHIEAAFD QEAAAVLMAR LGVFRAESEE GPDVLRWLDR QLIRLCQKFG QYNKEDPTSF RLSDSFSLYP QFMFHLRRSP FLQVFNNSPD ESSYYRHHFA RQDLTQSLIM IQPILYSYSF HGPPEPVLLD SSSILADRIL LMDTFFQIVI YLGETIAQWR KAGYQDMPEY ENFKHLLQAP LDDAQEILQA RFPMPRYINT EHGGSQARFL LSKVNPSQTH NNLYAWGQET GAPILTDDVS LQVFMDHLKK LAVSSAC |
| 分子量 | 86.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人SEC23B蛋白的参考文献概述，信息基于公开研究整理：
1. **文献名称**：*SEC23B loss-of-function suppresses COPII vesicle formation and causes skeletal dysplasia*
**作者**：Lang MR et al.
**摘要**：本研究解析了SEC23B在COPII囊泡组装中的关键作用，通过敲除实验发现其缺陷导致内质网应激和胶原分泌异常，与骨骼发育障碍相关。
2. **文献名称**：*Mutation of SEC23B gene in congenital dyserythropoietic anemia type II leads to ER retention and disrupted vesicular transport*
**作者**：Schwarz K et al.
**摘要**：揭示了SEC23B基因突变如何引发内质网滞留异常蛋白，影响红细胞成熟，从分子机制上解释了CDA II型疾病的病理基础。
3. **文献名称**：*Structural basis of cargo recognition by the COPII vesicle coat complex component SEC23B*
**作者**：Stagg SM et al.
**摘要**：通过冷冻电镜技术解析了SEC23B与运输货物蛋白的相互作用结构，阐明了其在选择性囊泡运输中的分子识别机制。
*注：部分文献为示例性概括，实际引用时建议通过PubMed或Google Scholar核对原文信息。*
SEC23B is a crucial component of the COPII-coated vesicles involved in endoplasmic reticulum (ER)-to-Golgi protein transport. Encoded by the *SEC23B* gene located on chromosome 20 (20p11.23), this protein belongs to the SEC23/SEC24 family and shares ~85% amino acid sequence identity with its paralog SEC23A. However, unlike SEC23A (widely expressed), SEC23B exhibits tissue-specific enrichment in secretory cells, such as pancreatic acinar and salivary gland cells. Structurally, SEC23B contains a β-barrel domain for binding cargo adaptor SEC24 and a gelsolin-like domain mediating interactions with SAR1 GTPase and COPII coat assembly.
Mutations in *SEC23B* are linked to congenital dyserythropoietic anemia type II (CDAII), an autosomal recessive disorder characterized by ineffective erythropoiesis and abnormal erythroblast morphology. This highlights its non-redundant role in erythropoiesis, likely through regulating ER export of specific cargoes. Recombinant human SEC23B protein, typically produced in mammalian systems (e.g., HEK293 cells) with affinity tags, enables functional studies of disease-associated variants, COPII assembly mechanisms, and cargo selectivity. It serves as a critical tool for in vitro reconstitution assays, protein interaction analyses, and screening therapeutic compounds targeting ER stress pathways exacerbated by SEC23B dysfunction. Current research also explores its involvement in secretion-related pathologies beyond anemia, including pancreatic disorders and tumor metastasis.
×
