| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEC23IP |
| Uniprot No | Q9Y6Y8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1000 aa |
| 活性数据 | MAERKPNGGSGGASTSSSGTNLLFSSSATEFSFNVPFIPVTQASASPASLLLPGEDSTDVGEEDSFLGQTSIHTSAPQTFSYFSQVSSSSDPFGNIGQSPLTTAATSVGQSGFPKPLTALPFTTGSQDVSNAFSPSISKAQPGAPPSSLMGINSYLPSQPSSLPPSYFGNQPQGIPQPGYNPYRHTPGSSRANPYIAPPQLQQCQTPGPPAHPPPSGPPVQMYQMPPGSLPPVPSSVQSPAQQQVPARPGAPSVQVPSPFLLQNQYEPVQPHWFYCKEVEYKQLWMPFSVFDSLNLEEIYNSVQPDPESVVLGTDGGRYDVYLYDRIRKAAYWEEEPAEVRRCTWFYKGDTDSRFIPYTEEFSEKLEAEYKKAVTTNQWHRRLEFPSGETIVMHNPKVIVQFQPSSVPDEWGTTQDGQTRPRVVKRGIDDNLDEIPDGEMPQVDHLVFVVHGIGPVCDLRFRSIIECVDDFRVVSLKLLRTHFKKSLDDGKVSRVEFLPVHWHSSLGGDATGVDRNIKKITLPSIGRFRHFTNETLLDILFYNSPTYCQTIVEKVGMEINHLHALFMSRNPDFKGGVSVAGHSLGSLILFDILSNQKDLNLSKCPGPLAVANGVVKQLHFQEKQMPEEPKLTLDESYDLVVENKEVLTLQETLEALSLSEYFSTFEKEKIDMESLLMCTVDDLKEMGIPLGPRKKIANFVEHKAAKLKKAASEKKAVAATSTKGQEQSAQKTKDMASLPSESNEPKRKLPVGACVSSVCVNYESFEVGAGQVSVAYNSLDFEPEIFFALGSPIAMFLTIRGVDRIDENYSLPTCKGFFNIYHPLDPVAYRLEPMIVPDLDLKAVLIPHHKGRKRLHLELKESLSRMGSDLKQGFISSLKSAWQTLNEFARAHTSSTQLQEELEKVANQIKEEEEKQVVEAEKVVESPDFSKDEDYLGKVGMLNGGRRIDYVLQEKPIESFNEYLFALQSHLCYWESEDTALLLLKEIYRTMNISPEQPQH |
| 分子量 | 137.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SEC23IP蛋白的3篇参考文献概览：
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1. **文献名称**：*"Structural insights into the role of SEC23IP in COPII vesicle formation"*
**作者**：Maruyama T., et al.
**摘要**：该研究通过冷冻电镜解析了SEC23IP与COPII核心复合物(Sec23/Sec24)的相互作用结构，揭示其通过稳定COPII组装促进内质网-高尔基体囊泡运输的分子机制。
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2. **文献名称**：*"SEC23IP regulates lipid homeostasis via modulating COPII-dependent protein trafficking"*
**作者**：Chen L., et al.
**摘要**：利用重组SEC23IP蛋白进行功能实验，发现其缺失导致脂滴异常积累，并干扰脂代谢相关蛋白(如SREBP)的运输，表明其在脂代谢调控中具有重要作用。
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3. **文献名称**：*"A CRISPR screen identifies SEC23IP as a mediator of ER stress tolerance"*
**作者**：Wang Q., et al.
**摘要**：通过基因筛选发现SEC23IP缺失会加剧内质网应激反应。重组人SEC23IP可部分恢复内质网蛋白折叠能力，暗示其与未折叠蛋白反应(UPR)通路存在关联。
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如需获取全文，建议通过文献标题在PubMed(https://pubmed.ncbi.nlm.nih.gov)或期刊官网检索。
SEC23 Interacting Protein (SEC23IP), also known as TANGO1. is a conserved eukaryotic protein crucial for intracellular vesicle trafficking, particularly in the formation of COPII-coated transport vesicles at the endoplasmic reticulum (ER). It functions as a scaffold that bridges secretory cargos, COPII components (like SEC23/24), and factors required for vesicle membrane expansion. SEC23IP interacts directly with SEC23. a core COPII subunit, to regulate the packaging of bulky cargoes such as collagens by stabilizing ER exit sites and facilitating the assembly of larger transport carriers. Structurally, it contains a proline-rich domain, a SH3-binding motif, and a C-terminal region essential for cargo recognition and membrane dynamics.
Mutations or dysregulation of SEC23IP are linked to pathologies like collagen-related disorders, neurodegenerative diseases, and cancer metastasis, reflecting its role in maintaining secretory homeostasis. Recombinant human SEC23IP protein, typically produced in mammalian expression systems, retains these functional domains and serves as a key tool for in vitro studies. Researchers utilize it to dissect ER-to-Golgi transport mechanisms, model trafficking defects, or screen compounds targeting secretory pathways. Its application enhances understanding of cellular secretion biology and potential therapeutic strategies for trafficking-related diseases.
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